ISSN:
1432-1424
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary The salt pump of the isolated rabbit gallbladder, which transports an isotonic fluid solution from the lumen of the organ to the serosal tissue spaces, utilizes an endogenous substrate source for the neutral transport of NaCl. Exogenous substrates (glucose, acetate) do not elevate transport rates above control values if added when the preparation is made, but exogenous substrates are utilized, as pumping is maintained at initial pumping rates for longer periods of time than without substrate. Substrate addition with the inclusion of bicarbonate ion is a stabilizing factor for thisin vitro preparation. Although bicarbonate ion leads to an increase in transport rate without exogenous substrate, the pumping rate declines with time. Oxygen consumption falls over long periods with continued bicarbonate presence. The bicarbonate effect persists in thein vitro preparation after 20 to 24 hr at 4°C. After such treatment, glucose stimulates fluid transport. Thus, bicarbonate ion alters the glucose utilization of the gallbladder mucosa and the channeling of energy for fluid transport.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01870113
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