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  • 1
    Digitale Medien
    Digitale Medien
    DNase I sensitivity of the chromatin of the yeast SUC2 gene for invertase (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 422-427 
    Details
    ISSN: 1617-4623
    Schlagwort(e): SUC2 gene ; Active chromatin ; DNase I hypersensitive sites ; Saccharomyces cerevisiae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The DNase I sensitivity of chromatin of the yeast SUC2 gene, which encodes two forms of invertase, has been studied both in the genome and in a multicopy plasmid carrying the gene and its flanking sequences. Whereas little if any difference in the DNase I sensitivity of the flanking regions was found between the repressed and the derepressed states, derepression of the gene was accompanied by a large increase in the sensitivity of the transcribed region. A well-defined DNase I hypersensitive site was found centered at ∼ 120 bp downstream from the end of the coding region. This site seems to be flanked in the 3′ non-coding region by strictly positioned nucleosomes, and the structure of this region changes upon derepression. In the 5′ non-conding region two DNase I hypersensitive sites have been found flanking the TATA box and a set of three closely spaced hypersensitive sites occurs in an upstream regulatory sequence. The structure of these latter sites depends on the on-off state of transcription.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00338077
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  • 2
    Digitale Medien
    Digitale Medien
    Chromatin structure of the yeast SUC2 promoter in regulatory mutants (1992)
    Springer
    Molecular genetics and genomics 231 (1992), S. 395-400 
    Details
    ISSN: 1617-4623
    Schlagwort(e): SUC2 ; Saccharomyces cerevisiae ; Chromatin ; Micrococcal nuclease ; Promoter
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have previously suggested that two positioned nucleosomes are removed from the promoter of the Saccharomyces cerevisiae SUC2 gene upon derepression by glucose starvation. To gain further insight into the changes accompanying derepression at the chromatin level we have studied the chromatin structure of the SUC2 promoter in several mutants affecting SUC2 expression. The non-derepressible mutants snf1, snf2 and snf5 present a chromatin structure characteristic of the repressed state, irrespective of the presence or absence of glucose. The non-repressible mutants, mig1 and ssn6, as well as the double mutant snfs sn6 exhibit an opened chromatin structure even in the presence of glucose. These results suggest that the DNA-binding protein encoded by MIG1 is necessary to produce the characteristic pattern of repressed chromatin and that the SNF1 protein kinase is sufficient to produce the derepressed chromatin pattern. A model is presented for the transitions that result in opening up of the chromatin structure.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00292708
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  • 3
    Digitale Medien
    Digitale Medien
    News & Notes: Stochastic Nucleosome Positioning in a Yeast Chromatin Region Is Not Dependent on Histone H1 (1999)
    Springer
    Current microbiology 39 (1999), S. 168-172 
    Details
    ISSN: 1432-0991
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. To gain a better understanding of the function of the yeast histone H1, its role in nucleosome positioning was studied. With this objective in mind, we analyzed a chromatin region of the yeast Chromosome (Chr) IX, in which there are two closely packed open reading frames (ORFs), POT1 and YIL161w. This locus shows a regular ladder of 13 stochastically positioned nucleosomes, which is unaffected by the absence of the HHO1 gene. This suggests that histone H1 has no effect on nucleosome positioning in yeast.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002849900440
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  • 4
    Digitale Medien
    Digitale Medien
    Differential pattern of trehalose accumulation in wine yeast strains during the microvinification process (1999)
    Springer
    Biotechnology letters 21 (1999), S. 271-274 
    Details
    ISSN: 1573-6776
    Schlagwort(e): microvinification ; trehalose ; wine yeast
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Trehalose accumulation in wine yeast strains growing under microvinification conditions was determined and compared to that obtained under laboratory conditions. Industrial strains accumulate 10-fold more trehalose than laboratory strains. Contrary to batch-culture growth, under microvinification conditions trehalose accumulation is not consequence of glucose exhaustion. Physiological relevance of trehalose during the process of wine making and their use for potential improvements of alcoholic fermentation are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1005456917283
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  • 5
    Digitale Medien
    Digitale Medien
    Transcriptional and Structural Study of a Region of Two Convergent Overlapping Yeast Genes (1999)
    Springer
    Current microbiology 39 (1999), S. 369-373 
    Details
    ISSN: 1432-0991
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. The exceptionally close packing of many yeast genes and other chromosomal elements raises the question of how those elements are functionally insulated. All published work shows that natural insulators are very effective, but transcriptional interference (TI) occurs if they are mutated or if their natural context is altered. Mechanisms to avoid TI are poorly understood, but are thought to involve an interplay of cis sequences and trans factors in a chromatin context. We have studied the case of two convergent closely packed ORFs (56 bp of separation) in chromosome IX of Saccharomyces cerevisiae. mRNAs from POT1 and YIL161w overlap by up to 115 nt. Convergent transcription causes a small but noticeable negative effect on the level of POT1 mRNA and nucleosome displacement in the intergenic region. This suggests for the first time that some TI could occur in convergently transcribed yeast genes, even in a natural chromosomal context.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002849900474
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  • 6
    Digitale Medien
    Digitale Medien
    A new glucose-repressible gene identified from the analysis of chromatin structure in deletion mutants of yeast SUC2 locus (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 7 (1991), S. 379-389 
    Details
    ISSN: 0749-503X
    Schlagwort(e): Chromatin ; SUC2 ; Glucose repression ; 3-Oxoacyl-CoA thiolase gene ; Saccharomyces cerevisiae ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: We have previously shown that some changes occur in the chromatin structure of the 3′ flank of the yeast SUC2 gene in going from a repressed to an active state. In an attempt to find out the causes of these changes, we have carried out experiments in which mutant copies of SUC2 locus lacking either 5′ or 3′ flanks have been analysed for their transcriptional activity and chromatin structure. These experiments allowed us to discard any relationship between SUC2 transcription and chromatin changes within its 3′ flank. Sequencing of this flank and mRNA analysis, however, resulted in the location of a putative peroxisomal 3-oxoacyl-CoA thiolase gene (POT1), which is repressible by glucose. The disruption of the gene produced a yeast strain unable to use oleic acid as a carbon source. This is the first time that chromatin structure analysis has permitted the identification of new gene.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/yea.320070408
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  • 7
    Digitale Medien
    Digitale Medien
    Chromatin structure of yeast genes (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 5 (1989), S. 219-238 
    Details
    ISSN: 0749-503X
    Schlagwort(e): Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/yea.320050404
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