ISSN:
0091-7419
Keywords:
α-actinin
;
plasma membranes
;
actin attachment
;
immunoautoradiography on gels
;
Life Sciences
;
Molecular Cell Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
The role of α-actinin in the attachment of actin to plasma membranes has been investigated. Specific antibody staining of SDS gels has indicated that α-actinin is a major component in isolated plasma membranes prepared from three different cell types by two different procedures. Using specific extraction conditions, most of the α-actinin can be selectively extracted from the membranes with relatively little parallel release of actin. This selective dissociation of α-actinin from the plasma membrane leads us to conclude that α-actinin is present in these membrane preparations, because it is bound to actin, and that α-actinin does not form a direct link between actin and the membrane.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jss.400130106
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