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  • 1
    Electronic Resource
    Electronic Resource
    Encapsulation of the thrombolytic enzyme, brinase, in photosensitized erythrocytes: a novel thrombolytic system based on photodynamic activation
    Amsterdam : Elsevier
    Journal of Photochemistry and Photobiology B: Biology 26 (1994), S. 193-196 
    Details
    ISSN: 1011-1344
    Keywords: Brinase ; Electroporation ; Erythrocytes ; Fibrinolytic ; Photodynamic therapy ; Thrombolytic
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/1011-1344(94)07037-7
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A rapid in situ, colorimetric assay for the determination of mammalian cell viability in alginate-immobilized and encapsulated systems (1996)
    Springer
    Bioprocess and biosystems engineering 15 (1996), S. 47-49 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A convenient, rapid colorimetric assay system has been developed in order to determine cell viability of populations of mammalian cells encapsulated using a poly-L-lysine/ alginate encapsulation system. The method is based on the use of 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and involves direct incubation of the capsules with the reagent. This is followed by direct solubilization of the resulting formazan product in dimethyl sulfoxide (DMSO). In order to demonstrate that this assay method may be generally applicable to alginate-based carrier systems, viability of cells immobilized in alginate beads, as distinct from encapsulation in the poly-L-lysine/alginate system, was also determined using the in situ method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435528
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Production of ethanol at 45°C on starch-containing media by mixed cultures of the thermotolerant, ethanol-producing yeast Kluyveromyces marxianus IMB3 and the thermophilic filamentous fungus Talaromyces emersonii CBS 814.70 (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 408-411 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The thermotolerant, ethanol-producing yeast strain, Kluyveromyces marxianus IMB3, was shown to produce ethanol at 45°C on starch-containing media supplemented with a crude amylase preparation derived from the thermophilic, filamentous fungus Talaromyces emersonii CBS 813.70. Ethanol production on media containing 4% (w/v) starch increased to a maximum of 15 g/l with 40 h, and this represented 74% of the maximum theoretical yield. Subsequent experimentation involving growth of both organisms in fermentations on starch-containing media (4% w/v) demonstrated that the mixed-culture system was capable of ethanol production at 45°C with maximum yields at 12 g/l obtained with 65 h. The advantages associated with ethanol production by this system are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050426
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Production of ethanol at 45°C on starch-containing media by mixed cultures of the thermotolerant, ethanol-producing yeast Kluyveromyces marxianus IMB3 and the thermophilic filamentous fungus Talaromyces emersonii CBS 814.70 (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 408-411 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The thermotolerant, ethanol-producing yeast strain, Kluyveromyces marxianus IMB3, was shown to produce ethanol at 45°C on starch-containing media supplemented with a crude amylase preparation derived from the thermophilic, filamentous fungus Talaromyces emersonii CBS 813.70. Ethanol production on media containing 4% (w/v) starch increased to a maximum of 15 g/l with 40 h, and this represented 74% of the maximum theoretical yield. Subsequent experimentation involving growth of both organisms in fermentations on starch-containing media (4% w/v) demonstrated that the mixed-culture system was capable of ethanol production at 45°C with maximum yields at 12 g/l obtained with 65 h. The advantages associated with ethanol production by this system are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218441
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on the use of a thermotolerant strain of Kluyveromyces marxianus in simultaneous saccharification and ethanol formation from cellulose (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 518-520 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was found to be capable of ethanol production during growth at 45°C on media containing milled paper and exogenously added commercial cellulase. At maximum achievable cellulose concentrations in shake-flask cultures, ethanol production increased to 6.6 g/l at 45°C, representing an overall level of conversion of 21% of the maximum theoretical yield. Subsequent studies involving variations in added cellulase concentrations to the batch systems demonstrated that ethanol yields could be increased to 10 g/l at 45°C, which represented 39% of the maximum theoretical yield. As a result of ethanol production at 45°C in the systems examined, we suggest that the thermotolerant ethanol-producing yeast strain K. marxianus represents a novel candidate for use in simultaneous saccharification and conversion of the resulting substrates to ethanol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218459
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Studies on the use of a thermotolerant strain of Kluyveromyces marxianus in simultaneous saccharification and ethanol formation from cellulose (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 518-520 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was found to be capable of ethanol production during growth at 45°C on media containing milled paper and exogenously added commercial cellulase. At maximum achievable cellulose concentrations in shake-flask cultures, ethanol production increased to 6.6 g/l at 45°C, representing an overall level of conversion of 21% of the maximum theoretical yield. Subsequent studies involving variations in added cellulase concentrations to the batch systems demonstrated that ethanol yields could be increased to 10 g/l at 45°C, which represented 39% of the maximum theoretical yield. As a result of ethanol production at 45°C in the systems examined, we suggest that the thermotolerant ethanol-producing yeast strain K. marxianus represents a novel candidate for use in simultaneous saccharification and conversion of the resulting substrates to ethanol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050444
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Studies on the biosorption of uranium by Talaromyces emersonii CBS 814.70 biomass (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 807-811 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Residual biomass, produced by the thermophilic fungus, Talaromyces emersonii CBS 814.70, following growth on glucose-containing media, was examined for its ability to take up uranium from aqueous solution. It was found that the biomass had a relatively high observed biosorption capacity for the uranium (280 mg/g dry weight biomass). The calculated maximum biosorption capacity obtained by fitting the data to a Langmuir model was calculated to be 323 mg uranium/g dry weight biomass. Pretreatment of the biomass with either dilute HCl or NaOH brought about a significant decrease in biosorptive capacity for uranium. Studies on the effects of variation in temperature on the biosorptive capacity demonstrated no significant change in binding between 20°C and 60°C. However, a significant decrease in biosorptive capacity was observed at 5°C. Binding of uranium to the bio mass at all temperatures reached equilibrium within 2 min. While the routine binding assays were performed at pH 5.0, adjustment of the pH to 3.0 gave rise to a significant decrease in biosorption capacity by the biomass. The biosorptive capacity of the biomass for uranium was increased when extraction from solution in sea-water was examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050334
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Studies on the biosorption of uranium by Talaromyces emersonii CBS 814.70 biomass (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 807-811 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Residual biomass, produced by the thermophilic fungus, Talaromyces emersonii CBS 814.70, following growth on glucose-containing media, was examined for its ability to take up uranium from aqueous solution. It was found that the biomass had a relatively high observed biosorption capacity for the uranium (280 mg/g dry weight biomass). The calculated maximum biosorption capacity obtained by fitting the data to a Langmuir model was calculated to be 323 mg uranium/g dry weight biomass. Pretreatment of the biomass with either dilute HCl or NaOH brought about a significant decrease in biosorptive capacity for uranium. Studies on the effects of variation in temperature on the biosorptive capacity demonstrated no significant change in binding between 20°C and 60°C. However, a significant decrease in biosorptive capacity was observed at 5°C. Binding of uranium to the biomass at all temperatures reached equilibrium within 2 min. While the routine binding assays were performed at pH 5.0, adjustment of the pH to 3.0 gave rise to a significant decrease in biosorption capacity by the biomass. The biosorptive capacity of the biomass for uranium was increased when extraction from solution in sea-water was examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171965
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    A rapid in situ, colorimetric assay for the determination of mammalian cell viability in alginate-immobilized and encapsulated systems (1996)
    Springer
    Bioprocess engineering 15 (1996), S. 47-49 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A convenient, rapid colorimetric assay system has been developed in order to determine cell viability of populations of mammalian cells encapsulated using a poly-L-lysine/ alginate encapsulation system. The method is based on the use of 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and involves direct incubation of the capsules with the reagent. This is followed by direct solubilization of the resulting formazan product in dimethyl sulfoxide (DMSO). In order to demonstrate that this assay method may be generally applicable to alginate-based carrier systems, viability of cells immobilized in alginate beads, as distinct from encapsulation in the poly-L-lysine/alginate system, was also determined using the in situ method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435528
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Growth of a thermotolerant ethanol-producing strain of Kluyveromyces marxianus on cellobiose containing media (1994)
    Springer
    Biotechnology letters 16 (1994), S. 625-630 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The thermotolerant yeast strain, Kluyveromyces marxianus 1MB 3, was shown to be capable of limited growth on cellobiose containing media at 45°C. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable fungal β-glucosidase. During active growth of the organism on cellobiose-containing media, β-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added β-glucosidase which results in increased substrate access to the biocatalytic unit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00128612
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    Paper (German National Licenses)
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