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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 48 (1964), S. 368-380 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Diese Arbeit ist eine Erweiterung vorhergehender Berichte über den Einfluß der Größe der Impfmenge auf das Wachstum von Aspergillus oryzae, wo u. a. festgestellt wurde, daß die beobachteten Effekte durch bestimmte Spurenelement-Zusammensetzungen aufgehoben werden. Es wurde nun festgestellt, daß Zugabe von Ca zu Substrat A 1 die „gewohnten” Effekte erhöht, während Cu diese verringert mit einer Tendenz zur Umkehrung. Werden geeignete Mengen Äthylendiamintetraessigsäure zu Substrat A 1 zugegeben, so werden Kulturen, die aus kleiner Impfmenge erwachsen sind, stärker in ihrer Wachstumsgeschwindigkeit und maximaler Ausbeute an Mycel gehemmt als Kulturen von großer Impfdichte, was einer Verstärkung der „gewohnten” Effekte gleich kommt. Diese Effekte werden umgekehrt, wenn die Glucose des Substrates A 1 durch Al(OH)3-Fällung gereinigt wird, jedoch nur wenn diese Behandlung bei neutraler oder alkalischer Reaktion erfolgt. Diese Umkehrung ist auf nahezu vollständige Entfernung von Zn und gleichzeitige Anwesenheit von Fe in Substrat A 1 zurückzuführen. Schon Zugabe sehr kleiner Mengen Zn (5 μg ZnSO4 · 7 H2O/l) zum gereinigten Substrat übt eine Wirkung aus, welche aber in einer Hemmung der Wachstumsgeschwindigkeit besteht. Höhere Zn-Konzentrationen sind nötig, um das Wachstum der Kulturen von großen und kleinen Impfmengen zu fördern. Die vorerwähnte Umkehrung der Phänomene wurde aufgehoben, wenn ein Substrat mit leichter Caramelisierung der Glucose angewendet wurde.
    Notes: Summary Further to earlier reports indicating that the effects of inoculum size on growth of Aspergillus oryzae in synthetic media are abolished by certain trace element compositions, it has now been shown that those effects can be made more pronounced, but can also be reversed, by varying the balance of available trace elements. Addition of Ca to substrate A 1 increases the “usual” effects of inoculum size whereas Cu abolishes them with a tendency for reversal. Supplementation of substrate A 1 with suitable amounts of ethylenediaminetetraacetic acid results in a stronger inhibition of small-inoculum cultures than large ones, thus increasing the “usual” effects of inoculum size. These effects are reversed if the sugar of substrate A 1 is purified by Al(OH)3 co-precipitation method, but only if the treatment is carried out at neutral or alkaline reaction. This reversal of the phenomena is due to an almost complete absence of Zn after purification and presence of Fe in substrate A 1. The Al(OH)3 treatment of the sugar results in substrates so poor in Zn that addition of as little as 5 μg ZnSO4 · 7 H2O/l has an effect. This influence, however, results in an inhibition of the growth rate, and higher concentrations of Zn are required in order to obtain stimulation with both large and small-inoculum cultures. The above-mentioned reversal of the phenomena was abolished in a substrate with slightly caramelized glucose.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 88 (1972), S. 119-126 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Conidiation of Aspergillus niger was studied in carbon-limited and nitrogen-limited chemostat culture. Under citrate-limitation conidiation intensity varied inversely with dilution rate. Conidiophores were less complex than in aerial conidiation and at high dilution rates conidia occasionally developed from modified hyphal tips. Conidiation was difficult to achieve under glucose-limitation. At the low dilution rates that allowed limited conidiation steady state could not be maintained due to onset of autolysis. At higher dilution rates when steady state was readily obtained conidiation did not occur. The maximum yield constants under citrate-limitation and glucose-limitation were respectively 0.145 and 0.4 mg dry weight/mg substrate, while the relative specific maintenance values were 0.045 and 0.018 mg substrate/mg dry weight/h. Under ammonium-limitation with citrate as the carbon source there was no conidiation. When nitrate became the limiting nitrogen source conidiophore initiation occurred but biomass production was low and wash-out occurred at D=0.034 h-1.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 20 (1964), S. 132-132 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé L'intensité de respiration d'Aspergillus oryzae est fortement diminuée par adsorption du mycélium à un filtre d'acétate de cellulose soigneusement nettoyé. Cet effet suggère que certaines substances d'une importance capitale dans le métabolisme sont éliminées de la surface des hyphes.
    Type of Medium: Electronic Resource
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