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Transplanted CNS stem cells form functional synapses in vivo (2000)

Auerbach, Jonathan M. ; Eiden, Maribeth V. ; McKay, Ron D. G.

Oxford, UK : Blackwell Science Ltd

European journal of neuroscience 12 (2000), S. 0

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ISSN: 1460-9568

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: An understanding of developmental mechanisms and new cell therapies can be achieved by transplantation into the nervous system. Multipotential stem cells have been isolated from the foetal and adult central nervous system (CNS). Immortalized and primary precursor cells integrate into the developing brain generating both neurons and glia as defined by immunological and morphological criteria. Here we show for the first time that in vitro-expanded CNS precursors, upon transplantation into the brains of rats, form electrically active and functionally connected neurons. These neurons exhibit spontaneous and evoked postsynaptic events and respond to focal glutamate application. Donor cells were grafted into the foetal hippocampus, and the amplitude and frequency of spontaneous synaptic events were monitored in the grafted cells in area CA1 for the first month of postnatal life. The formation of synapses onto grafted neurons indicates that grafted CNS stem cells can be used to study synaptic development in vivo and has important implications for clinical cell replacement therapies.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1460-9568.2000.00067.x

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Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells (2000)

Lee, Sang-Hun ; Lumelsky, Nadya ; Studer, Lorenz ; [et al.]

[s.l.] : Nature America Inc.

Nature biotechnology 18 (2000), S. 675-679

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ISSN: 1546-1696

Source: Nature Archives 1869 - 2009

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: [Auszug] Embryonic stem (ES) cells are clonal cell lines derived from the inner cell mass of the developing blastocyst that can proliferate extensively in vitro and are capable of adopting all the cell fates in a developing embryo. Clinical interest in the use of ES cells has been stimulated by studies ...