ISSN:
1420-908X
Keywords:
Adenylate cyclase
;
Protein kinase C
;
Histamine H2 receptor
;
Stomach
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The isoform of protein kinase C responsible for the inhibition of histamine-stimulated adenylate cyclase by the phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), has been investigated in a particulate fraction prepared from the human gastric cancer cell line HGT-1. Theα and∈ isoforms of protein kinase C were detected in HGT-1 cells and in a 40,000 × g particulate fraction by immunoblotting procedures. The inhibitory effect of TPA on histamine-stimulated adenylate cyclase was enhanced by the presence of Ca2+, but decreased in a concentrationdependent manner by anti-peptide antibody to protein kinase Cα, but not to protein kinase C∈. Addition of Ca2+ and TPA to the 40,000 × g particulate fraction stimulated the phosphorylation of the protein kinase C substrate myelin basic peptide 4–14. Protein kinase Cα is probably the isoform responsible for inhibition of histamine-stimulated adenylate cyclase in HGT-1 cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01793214
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