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  • 1
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The bacterial biota associated with the cuticle surface of healthy benthic samples of crustose nonarticulated coralline algae from the east coast of Tasmania (Australia) was examined by bacteriological cultivation and electron microscopy. In 32 samples studied, the viable count on Zobell's marine agar (supplemented with vitamins) was 3.3×106 bacteria g−1 wet wt. (range 2.9×104–2.7×107). Of 732 strains isolated from 16 out of 32 samples and identified to genus level,Moraxella was the predominant genus (66%). In contrast,Moraxella comprised only 11% of 217 strains isolated from benthic seawater samples collected at the same time as coralline algae. In 22 out of 32 algal samples examined by scanning electron microscopy, the total count was 1.6 × 107 bacteria g−1 wet wt. (range 5.1× 106–3.8×107); the major morphotype was cocco-bacilli (80%). Several environmental factors did not significantly influence the viable count or generic distribution, or the total count or morphotypic distribution of bacteria on the cuticle. These factors included geographical site, season, storage of samples in aquarium conditions, and the presence or absence of abalone from shells that the coralline algae encrusted. The microbiota, consisting mostly of the nonmotile bacterial genusMoraxella, appeared to be highly adapted to its calcerous plant host.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Marine biotechnology 1 (1999), S. 580-587 
    ISSN: 1436-2236
    Keywords: Key words: Thraustochytrids, polyunsaturated fatty acid, biomass, commercial culture, microheterotroph
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract: Thraustochytrids are common marine microheterotrophs, taxonomically aligned with heterokont algae. Recent studies have shown that some thraustochytrid strains can be cultured to produce high biomass, containing substantial amounts of lipid rich in polyunsaturated fatty acid (PUFA). It is also evident that cell yield and PUFA production by some thraustochytrid strains can be varied by manipulation of physical and chemical parameters of the culture. At present, fish oils and cultured phototrophic microalgae are the main commercial sources of PUFA. The possible decline of commercial fish stocks and the relatively complex technology required to commercially produce microalgae have prompted research into possible alternative sources of PUFA. The culture of thraustochytrids and other PUFA-producing microheterotrophs is seen as one such alternative. Indeed, several thraustochytrid-based products are already on the market, and research into further applications is continuing. Many fish and microalgal oils currently available have relatively complex PUFA profiles, increasing the cost of preparation of high-purity PUFA oils. In contrast, some of the thraustochytrids examined to date have simpler PUFA profiles. If these or other strains can be grown in sufficient quantities and at an appropriate cost, the use of thraustochytrid-derived oils may decrease the high expense currently involved with producing high-purity microbial oils. As more is learned about the health and nutritional benefits of PUFA, demand for PUFA-rich products is expected to increase. Results to date suggest that thraustochytrids could form an important part in the supply of such products.
    Type of Medium: Electronic Resource
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