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  • 1
    Digitale Medien
    Digitale Medien
    Genes overexpressed in the transition zone from normal to tumor tissue in larynx and pharynx carcinoma (2001)
    [s.l.] : Nature Publishing Group
    Nature genetics 27 (2001), S. 52-52 
    Details
    ISSN: 1546-1718
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Medizin
    Notizen: [Auszug] To identify genes relevant in the development and progression of larynx and pharynx carcinoma, we investigated samples derived from the transition zone between normal tissue and tumor. For larynx and pharynx carcinoma this transition zone offers an ideal starting point to identify genes involved in ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/87068
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  • 2
    Digitale Medien
    Digitale Medien
    An almost-intact human endogenous retrovirus K on human chromosome 7 (1999)
    [s.l.] : Nature America Inc.
    Nature genetics 21 (1999), S. 257-258 
    Details
    ISSN: 1546-1718
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Medizin
    Notizen: [Auszug] I n contrast to the many defective endoge- nous retroviral sequences, the human endogenous retrovirus HERV-K(HML-2) (ref. 1) group shows conservation of intact retroviral genes (for review, see ref. 2). We have previously reported evidence that human chromosome 7 contains both intact HERV-K gag ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/6766
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  • 3
    Digitale Medien
    Digitale Medien
    Recombination Multiply infected spleen cells in HIV patients (2002)
    [s.l.] : Nature Publishing Group
    Nature 418 (2002), S. 144-144 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] The genome of the human immunodeficiency virus is highly prone to recombination, although it is not obvious whether recombinants arise infrequently or whether they are constantly being spawned but escape identification because of the massive and rapid turnover of virus particles. Here we use ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/418144a
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  • 4
    Digitale Medien
    Digitale Medien
    Debrisoquine hydroxylase gene polymorphism in meningioma (1994)
    Springer
    Acta neuropathologica 88 (1994), S. 472-474 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Meningioma ; Detoxification gene Mutation ; Polymerase chain reaction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Cytochrome P450 CYP2D6 polymorphism is an autosomal recessive trait associated with impaired debrisoquine metabolism in 5–10% of caucasian populations. This polymorphism has been associated with susceptibility to Parkinson's disease, bladder cancer, various forms of leukemia and possibly melanoma. In many other cancer forms, the data remained contradictory due to the technical limitations for identifying affected individuals (poor metabolizers). A recently developed polymerase chain reaction-based assay allows convenient screening of approximately 80% of known mutations. We have tested brain tumors correlated with chromosome 22 deviations for genetic polymorphism in the cytochrome P450 CYP2D6 locus localized on chromosome 22q13. Thirty-one meningioma samples were analyzed and the observed frequency of heterozygotes and homozygotes for the G to A mutation did not deviate significantly from the distribution in a normal population. These data are comparable to previous observations in for example breast and colon cancer and indicate that the CYP2D6 locus on chromosome 22q13 is not involved in the pathogenesis of meningiomas.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00389501
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  • 5
    Digitale Medien
    Digitale Medien
    Debrisoquine hydroxylase gene polymorphism in meningioma (1994)
    Springer
    Acta neuropathologica 88 (1994), S. 472-474 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words Meningioma ; Detoxification gene ; Mutation ; Polymerase chain reaction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Cytochrome P450 CYP2D6 polymorphism is an autosomal recessive trait associated with impaired debrisoquine metabolism in 5 – 10   % of caucasian populations. This polymorphism has been associated with susceptibility to Parkinson's disease, bladder cancer, various forms of leukemia and possibly melanoma. In many other cancer forms, the data remained contradictory due to the technical limitations for identifying affected individuals (poor metabolizers). A recently developed polymerase chain reaction-based assay allows convenient screening of approximately 80   % of known mutations. We have tested brain tumors correlated with chromosome 22 deviations for genetic polymorphism in the cytochrome P450 CYP2D6 locus localized on chromosome 22q13. Thirty-one meningioma samples were analyzed and the observed frequency of heterozygotes and homozygotes for the G to A mutation did not deviate significantly from the distribution in a normal population . These data are comparable to previous observations in for example breast and colon cancer and indicate that the CYP2D6 locus on chromosome 22q13 is not involved in the pathogenesis of meningiomas.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00389501
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  • 6
    Digitale Medien
    Digitale Medien
    Coamplification on chromosomes 7p12-13 and 9q12-13 identified by reverse chromosome painting in a glioblastoma multiforme (1994)
    Springer
    Human genetics 〈Berlin〉 93 (1994), S. 331-334 
    Details
    ISSN: 1432-1203
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract DNA amplification is known to occur in approximately 50% of glioblastomas, with the epidermal growth factor receptor (EGFR) gene being the most frequently amplified. Whereas previous amplification studies have largely been limited to the analysis of known tumor-related genes, reverse chromosome painting allows us to search for as yet unidentified amplified domains. Here, we report the analysis of a glioblastoma multiforme by reverse chromosome painting. Hybridization signals were found on chromosome 7p12-13 and chromosome 9q12-13. Standard Southern blot analysis revealed amplification of the EGFR gene, which is localized on band 7p13. These findings corroborate previous reports on coamplification of sequences on different chromosomes in glioblastoma.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00212033
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  • 7
    Digitale Medien
    Digitale Medien
    Generation of a chromosome-22-specific c-DNA library as confirmed by FISH analysis (1993)
    Springer
    Human genetics 〈Berlin〉 92 (1993), S. 623-626 
    Details
    ISSN: 1432-1203
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract We have recently developed a strategy for the rapid enrichment of c-DNA fragments from selected human chromosomes. Heteronuclear RNA (hn-RNA) is isolated from a somatic cell hybrid that retains a single human chromosome in a rodent background. Following c-DNA synthesis, human sequences are selectively amplified by the Alu polymerase chain reaction (Alu-PCR). Here we have applied this protocol for the selective isolation of novel c-DNAs encoded by chromosome 22. Fluorescence in situ hybridization has been used to confirm the chromosome-22-specific origin of the c-DNA fragments. Controls show DNAse-free RNase-treated hn-RNA results in no c-DNAs or Alu-PCR products. As demonstrated by competitive in situ suppression hybridization (CISS), the majority of the Alu-PCR products from hybrid GM 10027 are located on chromosome 22. Without competition, hybridization signals have also been identified on other human chromosomes. These unspecific hybridization signals result from Alu sequences and can successfully be reduced by competition with cot 1 DNA. This is the first report of the use of CISS for the localization of chromosome-specific c-DNAs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00420950
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  • 8
    Digitale Medien
    Digitale Medien
    Chromosome specific c-DNA libraries: reduction of unspecific priming events by purification of heteronuclear RNA (1994)
    Springer
    Molecular biology reports 19 (1994), S. 89-92 
    Details
    ISSN: 1573-4978
    Schlagwort(e): hnRNA ; hncDNA ; Alu ; human chromosomes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Chromosome specific c-DNA libraries greatly facilitate the isolation of disease associated genes which have been previously linked to particular chromosomes. Recently, several methods have been developed and employed for the isolation of transcribed sequences from specific human chromosomes and chromosome regions. Heteronuclear (hn) RNA from somatic human/rodent cell hybrids has been used as starting material to selectively prime the synthesis of human specific c-DNAs. A drawback of this method is the high number of rodent clones found in these chromosome specific c-DNA libraries. Here, we provide direct evidence that unspecific priming events account for the majority of these rodent clones. Using an Alu consensus primer hn-RNA human specific c-DNA libraries have been established and the specificity of Alu-priming has been evaluated. Using a variety of purification schemes for isolating hn-RNA we have significantly reduced the percentage of unspecific priming events. We also included a comparison of the hn-RNA yield from different somatic hybrids prior and after purification.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00997152
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  • 9
    Digitale Medien
    Digitale Medien
    Rapid step-gradient purification of mitochondrial DNA (1988)
    Springer
    Molecular biology reports 13 (1988), S. 117-120 
    Details
    ISSN: 1573-4978
    Schlagwort(e): Mitochondria ; extrachromosomal DNA ; CsCl gradient ; DNA label ; restriction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A convenient modification of the step gradient (CsCl/ethidium bomide) procedure is described. This rapid method allows isolation of covalently closed circular DNA separated from contaminating proteins, RNA and chromosomal DNA in ca. 5 h. Large scale preparations can be performed for circular DNA from eukaryotic organelles (mitochondria). The protocol uses organelle pelleting/NaCl-sarcosyl incubation steps for mitochondria followed by a CsCl step gradient and exhibits yields equal to the conventional procedures. It results in DNA sufficiently pure to be used for restriction endonuclease analysis, subcloning, 5′-end labeling, gel retention assays, and various types of hybridization.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00539059
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  • 10
    Digitale Medien
    Digitale Medien
    Enrichment of chromosome specific hncDNAs by magnetic bead coupled Alu sequences (1995)
    Springer
    Molecular biology reports 22 (1995), S. 53-57 
    Details
    ISSN: 1573-4978
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have employed a strategy for the rapid enrichment of cDNA clones from human chromosome 22 utilizing magnetic beads. Starting from a somatic cell hybrid which retains chromosome 22 in rodent background, heteronuclear (hn) RNA was transcribed into hncDNA using poly dT-primers. Using linker specific primers hncDNA was amplified by PCR. To identify chromosome 22 specific hncDNAs a highly human specific Alu consensus sequence (PD39) was biotinylated and hybridized to the PCR product of the hncDNAs in solution. Hybridized hncDNA-PD39 complexes are captured using streptavidin-coated magnetic beads. Hybridized hncDNAs are selectively amplified by PCR. To verify the chromosome specificity the hncDNA was used as probe forin situ hybridization. Following two rounds of selection with magnetic beads there was an increasingly strong hybridization signal on chromosome 22. The capturing of hncDNAs by magnetic beads as described in this study is faster and more efficient than previously described methods for the isolation of chromosome specific hncDNAs. The novel approach has been employed to generate hncDNAs highly enriched for chromosome 22 specific sequences.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00996305
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