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  • 1
    ISSN: 1432-1041
    Keywords: Key words Fluvastatin ; LDL oxidation; plasma trace metals ; α-tocopherol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Objective: Oxidation of low density lipoproteins (LDL) is thought to be an important step in the development of atherosclerosis. Trace metals are involved in oxidative processes. It was of interest to determine whether lipid-lowering therapy with fluvastatin, a potent HMGCoA reductase inhibitor, affected LDL oxidation and trace metal levels. Methods: Twenty hypercholesterolemic patients were treated with fluvastatin (40 mg twice daily) or placebo for 8 weeks in a double-blind, randomized study. LDL composition, antioxidants and oxidizability as well as plasma zinc, copper, selenium and manganese concentrations were investigated. Results: After fluvastatin treatment, total cholesterol was reduced by 24%, triglycerides fell by 26% and plasma Zn fell by 8%. Cu, Se and Mn changed insignificantly. LDL were separated by ultracentrifugation. LDL were reduced by 18%, LDL-C by 29% and LDL-TG by 19%. The concentrations of α-tocopherol and retinol in LDL changed insignificantly. LDL preparations were incubated with copper ions (204 μmol · l−1 LDL-C/3.2 μmol · l−1 Cu) and formation of conjugated dienes was monitored at 234 nm for 5 h. Treatment with fluvastatin caused a reduction of diene production by 16% and of diene production rate by 14%, effects being significantly different from placebo (P=0.02). The change of the lagtime did not reach significance; however, it was positively correlated with the change in LDL α-tocopherol. In the placebo group, no significant effects were observed. Conclusion: Fluvastatin therapy had lipid-lowering and antioxidative effects.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Chemical Physics Letters 96 (1983), S. 34-37 
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Chemical Physics Letters 127 (1986), S. 419-423 
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 151 (2004), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  Variegate porphyria (VP) is an autosomal dominant disorder associated with deficient haem synthesis. Recent reports indicate that the clinical penetrance of VP may have been overestimated in studies which predated the availability of DNA-based testing for VP.Objectives  To undertake a study specifically designed to assess the clinical and biochemical penetrance of VP in a kindred characterized by gene status.Methods  We studied a large family carrying the South African founder mutation which is known to result in almost complete haplodeficiency. All informative members were tested for the R59W mutation. Biochemical evidence of porphyria was sought by porphyrin analysis and by plasma fluorescence scanning. The presence of clinically expressed porphyria was assessed using a structured questionnaire and telephone or personal interview.Results  Of 62 informative subjects, 33 had inherited the mutation. Of 28 adults, one subject had experienced a single acute attack. She and a further 10 subjects had experienced photosensitivity. The frequency of acute attacks in this family is therefore 4% (95% confidence interval, CI 1–18%), and of photosensitivity is 39·3% (95% CI 24–58%). The sensitivity and specificity of porphyrin analysis in this family were 0·46 (95% CI 0·30–0·64) and 1·00 (95% CI 0·85–1·00), respectively, and for plasma scanning the values were 0·85 (95% CI 0·58–0·96) and 1·00 (95% CI 0·72–1·00), respectively.Conclusions  The clinical penetrance of VP in our family is approximately 40%. Many more subjects with VP are diagnosed in an asymptomatic phase than previously, and the acute attack is now an uncommon manifestation of VP. Plasma scanning is more sensitive than faecal porphyrin analysis, but neither is sufficiently sensitive for the detection of carrier status.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Electrochimica Acta 37 (1992), S. 799-809 
    ISSN: 0013-4686
    Keywords: charge transfer. ; photoelectrochemistry kinetics ; semiconductor electrode
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Ultramicroscopy 42-44 (1992), S. 1403-1411 
    ISSN: 0304-3991
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Electroanalytical Chemistry 214 (1986), S. 685-698 
    ISSN: 0022-0728
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Electroanalytical Chemistry 228 (1987), S. 45-53 
    ISSN: 0022-0728
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Applied Surface Science 27 (1987), S. 423-436 
    ISSN: 0169-4332
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1434-5196
    Keywords: Key words DNA typing ; Epithelial cells ; Physical ; violence ; Schlüsselwörter DNA-Typisierung ; Hautzellen ; Körperliche Gewalt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Bei massiver körperlicher Gewalt mit Kontakt ungeschützter Hautflächen (Schlagen, Greifen) kam es im Probandenversuch zur wechselseitigen Behaftung mit Hautzellen, davon überwiegend Korneozyten und vereinzelt kernhaltige Zellen des Stratum granulosum. Schlagversuche auf folienbedeckte Sandsäcke führten zu einem gleichartigen Zellabtrag. Nach Faustschlägen auf die Oberarme gelang in einigen Fällen eine „Täter-Opfer-Zuordnung“über DNA-Typisierung. Die DNA stammte nicht nur aus den wenigen in der Spur enthaltenen Zellkernen sondern auch aus den Korneozyten selbst, wie Untersuchungen sicher kernfreier Zellsuspensionen ergaben. Die Größenordnungen der im Modellversuch in der Spur erreichten Zellzahlen zeigten jedoch, daß der Wert einer derartigen Spur durch das Vorhandensein kernhaltiger Zellen bestimmt wird. Die vorliegenden Ergebnisse rechtfertigen die Forderung, bei entsprechend gelagerter Kasuistik, zusammen mit der Kriminalpolizei, möglichst umgehend Abstriche der entsprechenden Hautregionen vorzunehmen.
    Notes: Abstract As a result of physical violence to exposed regions of skin (beating, grabbing) it was possible to effect a mutual transfer of epithelial cells between probands. The majority of these cells were corneocytes with a minimal incidence of nucleated stratum granulosum cells. Punching onto foil-covered sandbags resulted in a comparable transfer of cells. Punches afflicted to the upper arms resulted in a successful ‘perpetrator-victim’ assignment via DNA typing. The DNA did not only originate from the few nucleated cells but also from the corneocytes themselves, as positively confirmed by investigations of denucleated cell-suspensions. The cell concentration achieved in model experiments, however, shows that the forensic value of such a sample is dependent on the presence of nucleated cells. The results of this paper justify the requirement – (having weighed up the appropriate case-specific modalities) – that sampling by taking swabs of the skin regions in question must be carried out as soon as possible.
    Type of Medium: Electronic Resource
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