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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 280 (1979), S. 158-160 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Single muscle fibres from the M. semitendinosus of the frog (Rana tempor¡ria) were transferred into a Perspex chamber7 which allowed a quick exchange of solutions. Isometric contraction was measured with a force transducer5. To prevent a decrease in resting potential5'6 and an increase in external ...
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: Ca2+ release ; Sarcoplasmic reticulum ; Slow Ca2+ current ; Skeletal muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ca2+ channels are regulated in a variety of different ways, one of which is modulation by the Ca2+ ion itself. In skeletal muscle, Ca2+ release sites are presumably located in the vicinity of the dihydropyridine-sensitive Ca2+ channel. In this study, we have tried to investigate the effects of Ca2+ release from the sarcoplasmic reticulum on the L-type Ca2+ channel in frog skeletal muscle, using the double Vaseline gap technique. We found an increase in Ca2+ current amplitude on application of caffeine, a well-known potentiator of Ca2+ release. Addition of the fast Ca2+ buffer BAPTA to the intracellular solution led to a gradual decline in Ca2+ current amplitude and eventually caused complete inhibition. Similar observations were made when the muscle fibre was perfused internally with the Ca2+ release channel blocker ruthenium red. The time course of Ca2+ current decline followed closely the increase in ruthenium red concentration. This suggests that Ca2+ release from the sarcoplasmic reticulum is involved in the regulation of L-type Ca2+ channels in frog skeletal muscle.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of muscle research and cell motility 18 (1997), S. 353-367 
    ISSN: 1573-2657
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The time course of activation of theskeletal muscle l-type calcium channel was studied involtage-clamped myotubes derived from human satellite cells. Theslow l-type current was isolated by inactivating fastercalcium current components using appropriate prepulses or bysubtracting the currents not blocked by 5 μm nifedipine. Thel-type current exhibited a single exponential activation andtime constants which showed little voltage dependence in therange +10 to +50 mV. Currents blocked by nifedipine could bepartially restored by UV-light flash photolysis. When a flash oflight was applied during a depolarizing step, the activation timecourse of the resulting inward current contained a rapid, almostinstantaneous component followed by a slower component. Theamplitude of the rapid component was different when the flash wasapplied at different times during the depolarizing step:depolarization first increased and then decreased the fraction ofchannels which could rapidly be restored from the block byphotolysis. Plotted versus time after the onset of thedepolarization this fraction closely matched the time course ofthe l-type current obtained before the block by nifedipine.This indicates that the slow gating recations of the Ca2+ channelremain functional in the nifedipine-blocked state. Largeconditioning depolarizations which had been shown to enhance thespeed of l-type current activation in frog muscle fibresshowed no effect in human myotubes. Numerical simulations using agating scheme proposed for frog muscle demonstrate that suchdifferences can be caused by changing just a single kineticparameter
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 61 (1989), S. 964-967 
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aufgrund behördlicher Auflagen hat die Bestimmung toxischer Gasspuren in den letzten Jahren steigende Bedeutung erlangt. Schwierigkeiten ergeben sich sowohl bei der Entnahmetechnik als auch bei der Messung selbst. Anhand einiger ausgewählter Beispiele werden die auftretenden Probleme und die Wege zu ihrer Lösung im Detail erläutert. Dabei handelt es sich um die Messung chlorierter Kohlenwasserstoffe bei einer Raumluft-überwachung, die Bestimmung von Chlor im Abgas einer Abgaswäsche und die Messung von Phosgen-Spuren bei einer Raumluft- und Emissions-überwachung. Die Arbeit schließt mit einer Zusammenstellung wesentlicher Gesichtspunkte bei der Planung und Wartung von Gasspuren-Meßanlagen.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 61 (1989), S. 288-291 
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: State of technology and trends in the process analyzer field. The use of process analyzers is of growing importance in the chemical industry. For demonstration purpose this publication first describes some applications of analyzers in various fields of chemical production, and then surveys commercially available process analyzers and peripheral equipment. Market gaps are indicated. Problems in relating to μ-processor applications and electromagnetic interference are mentioned and trends in the process analyzer field are discussed.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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