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  • 1
    Electronic Resource
    Electronic Resource
    Interaction of monoclonal antibodies with the IgE-receptor on rat mast cells and rat basophilic leukemia (RBL) cells (1986)
    Springer
    Inflammation research 17 (1986), S. 418-426 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The close relation between rat mast cells and rat basophilic leukemia (RBL) cells with regard to the presence of receptors for IgE and Fcγ led us to generate monoclonal antobodies directed against cell surface antigens. Hybridomas were obtained by the fusion of NS1 mouse myeloma cells with murine spleen and lymph node cells. The culture supernatants were assayed by two ELISA techniques: a) for the production of mouse immunoglobulin in general and b) for antibodies directed against surface antigens of RBL cells. For this purpose RBL cells were attached to polyvinyl chloride microtitre plates. Eight hybrids produced antibodies directed against surface antigens on RBL cells. Hybrids were cloned and characterized with regard to their isotype and light chains. All eight clones secreted IgM with K light chains. Immunofluorescence studies performed with RBL cells revealed that all eight antibodies were able to show a specific fluorescence. Furthermore, four of these eight antibodies also showed a specific fluorescence with purified rat mast cells. These four antibodies were analyzed as to their ability of interacting with the IgE-receptor on RBL cells and purified rat mast cells. They reduced the binding rate of radiolabelled rat IgE to RBL and rat mast cells. A mutual inhibition of the passive cutaneous anaphylaxis (PCA) reaction in the rat by either mixing mouse reaginic serum directed against 2,4-dinitrophenol bovine serum albumin (DNP-BSA) or by mixing monoclonal mouse anti-DNP IgE with the monoclonal mouse anti-cell surface (rat basophilic leukemia, rat mast cell) IgM was determined. The histamine release of rat mast cells obtained from Nippo-strongylus brasiliensis infected rats was markedly reduced in the presence of two monoclonal anti cell surface antibodies. Our data suggest that monoclonal antibodies with specificity for the IgE receptor (inhibition of IgE-binding) can be distinguished from those which bind closely to the IgE-receptor but also modulate the histamine release from presensitized mast cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01965508
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Esophageal dysfunction in diabetes mellitus: is there a relation to clinical manifestation of neuropathy? (1992)
    Springer
    Journal of molecular medicine 70 (1992), S. 740-747 
    Details
    ISSN: 1432-1440
    Keywords: Diabetes mellitus ; Esophageal motility ; Manometry ; Autonomic cardiac neuropathy ; Peripheral sensorimotor neuropathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In a prospective study, we evaluated 33 diabetic patients [type I (n = 8) and type II (n = 25)]. Esophageal motor functions were examined by registering clinical symptoms and by performing esophageal manometry. We also investigated peripheral and autonomic neuropathy. In diabetics, the lower and upper esophageal sphincter pressure and amplitudes of peristaltic waves were reduced. Compared with controls (n = 30), in diabetics the esophageal peristaltic velocity was reduced significantly, and the duration of contractions were decreased as well. Multipeaked waves were uncommon in diabetics, while non-propulsive contractions were seen more often. No correlation was found between esophageal dysfunction and peripheral or autonomic neuropathy. Some 60% of diabetics reported esophageal symptoms; however, no relationship between these symptoms and the extent of dysfunction in esophageal motility was found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00180740
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Leberfunktion von Patienten mit arterieller Hypoxämie bei chronisch obstruktiver Atemwegserkrankung und der Einfluß der nasalen Sauerstoffinsufflation (1989)
    Springer
    Journal of molecular medicine 67 (1989), S. 833-838 
    Details
    ISSN: 1432-1440
    Keywords: Chronic obstructive airway disease ; Arterial hypoxemia ; Liver function ; Galactose elimination capacity ; Indocyaninegreen clearance ; Oxygen therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Patients suffering from chronic obstructive airway disease often have arterial hypoxemia, which is more or less severe. If hypoxemia deteriorates the function of the liver is still unknown. The aim of this study was to determine, if a mostly oxygen dependent process of liver metabolism (galactose elimination capacity) is disturbed and if it can be increased by oxygen insufflation. Under the same conditions an oxygen independent process (indocyaninegreen clearance) should not be influenced. Our results show, that galactose elimination capacity under room air was pathologic and can be increased significantly by oxygen breathing. No change of indocyaninegreen clearance was seen under the same conditions. After termination of oxygen therapy the galactose elimination capacity was as bad as before oxygen breathing. Dysfunctions of the liver, which are caused by hypoxemia, can be positively influenced by oxygen, but only for the duration of oxygen insufflation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01725200
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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