ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Aspergillus oryzae was grown in a minimal medium supplemented with nine different carbon sources. α-Amylase formation was induced by starch and maltose. With glucose, no carbon catabolite repression was observed, but surprisingly α-amylase production was induced; all other tested carbon sources failed to influence α-amylase synthesis. Simultaneous cultivation with two different carbon sources confirmed this α-amylase regulation. Northern blot analysis showed that α-amylase gene expression was controlled at the level of transcription. For further characterization of α-amylase expression,Aspergillus nidulans was transformed by theamyl gene ofA. oryzae. The regulation of α-amylase formation in three analyzed transformants by different carbon sources was comparable to that of theA. oryzae wildtype.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01577242
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