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  • 1
    Digitale Medien
    Digitale Medien
    Freeze-drying and embedding in glycol methacrylate (GMA) (1974)
    Springer
    Histochemistry and cell biology 39 (1974), S. 313-326 
    Details
    ISSN: 1432-119X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary 1. The well-known disadvantages in the use of freeze-dried-paraffin-embedded tissues, such as loss and displacements of tissue elements as well as difficulties in flattening sections can be prevented by embedding in glycol methacrylate (GMA). 2. Usual histological stainings and the histochemical demonstration of RNA, DNA and glycogen can be performed without removing the plastic material. The activity of acid and alkaline phosphatase, ATPase and 5-nucleotidase can be demonstrated without difficulty. 3. Lysosomal ali-esterases can be demonstrated with the indoxyl acetate method, while the E-600-sensitive microsomal esterases do react extremely weak. The reactivity of succinodehydrogenase is abolished. Immunohistological reactions to reveal human IgG are strongly diminished.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00495682
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    The fate of injected human IgG in the mouse liver (1975)
    Springer
    Cell & tissue research 156 (1975), S. 359-376 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Mouse-liver ; Human-IgG ; Lysosomes ; Fluorescent antibody technique ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary 1. Human IgG is stored in the form of remarkable droplets in the cytoplasm of mouse hepatocytes following intravenous injection. 2. The earliest protein uptake occurs within 1 minute after injection and droplets can be found up to 32 hours thereafter; 64 hours p.i. the foreign protein is no longer visible in hepatocytes. 3. Electron microscopy reveals that the uptake occurs by a process of “macropinocytosis”. The resulting protein droplets fuse with primary lysosomes and are transformed into phagolysosomes. 4. At the same time as the phagolysosomes are formed the IgG-droplets loose their immunological activity-as early as 2 hours after injection.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225364
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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