ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
A parental (CBAT6T6) lymph node cell suspension was injected into the hind footpad of F1 (CBA × C57BLKs) recipients. The resulting increase in the recipient's popliteal lymph node weight was linearly related on double log plots to the number of parental lymph node cells injected. The node weight response was biphasic including an initial rise, a maximum weight gain at seven to nine days and a gradual decline to normal weight. When the long-lived small lymphocyte population of the donor was labeled with 3H-thymidine, the location, morphology and mitotic activity of labeled cells was followed by radioautography. These cells were most frequently observed in the mid- and deep cortex and the medullary cords of the host node. Very few labeled blast cells were observed in sections or smears and radioautographs of chromosome spreads confirmed the infrequency of donor long-lived small lymphocyte transformation and proliferation. Of the proliferating cells in the host node at the peak of the response, 60-85% were of host origin. These observations and others are discussed, and it is proposed that the host short-lived small lymphocyte accounts for the majority of mitotic cells in the node at the peak of the response.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091790103
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