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  • 1
    Electronic Resource
    Electronic Resource
    Localization of mRNA Coding for the Three Subtypes of Atrial Natriuretic Factor (ANF) Receptors in Rat Anterior Pituitary Gland Cells (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neuroendocrinology 7 (1995), S. 0 
    Details
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Atrial Natriuretic Factor (ANF) action is mediated by highly selective and specific receptors. Three subtypes have been characterized and cloned: ANF receptor-A, -B and -C. These subtypes are all expressed in the anterior pituitary of the rat. In the present study, the mRNA for each subtype was detected by in situ hybridization. The amounts of ANFR-A and -B mRNA were found to be similar, and to be twice that of ANFR-C mRNA. At the ultrastructural level, the three types of ANFR mRNA were expressed in three anterior pituitary cell types, namely lactotrophs, corticotrophs, and gonadotrophs, identified by their hormonal content. No signal was revealed in somatotrophs or thyrotrophs. The different forms of mRNA were similar in terms of subcellular localization: in the cytoplasmic matrix and the nuclear euchromatin.These data indicate that the anterior pituitary is an important target tissue for ANF action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2826.1995.tb00739.x
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of endogenous osteocalcin in neonatal rat bone and its absence in articular cartilage: Effect of warfarin treatment (1990)
    Springer
    Virchows Archiv 417 (1990), S. 505-512 
    Details
    ISSN: 1432-2307
    Keywords: Osteocalcin ; Osteoblast ; Osteocyte ; Ultrathin frozen sections ; Warfarin treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunocytochemistry after cryoultramicrotomy was used to localize endogenous osteocalcin in bone (calvaria, femoral diaphysis) and epiphyseal femoral cartilage from 8-day-old rats treated (or mot) for 7 days with warfarin. Ultrathin frozen sections were incubated with goat antiserum against rat osteocalcin at high dilutions (2×10−4 to 2×10−6). In calvaria and femur of untreated rats, endogenous osteocalcin was observed in osteoblasts (cytoplasm and nucleus) and in the collagenous matrix. Osteocalcin appeared progressively in osteoblasts and bone matrix in the mineralization front, then increased in the regions of extended calcification. Osteocalcin was also detected in osteocytes but was not as abundant as in osteoblasts. In bone samples of warfarin-treated rats, endogenous osteocalcin was only detected in bone matrix but not in osteoblasts. Furthermore, osteocalcin was only observed if antiserum was not very dilute (2×10−2). In cartilage (hypertrophied and degenerative zones), osteocalcin was not observed in matrix and chondrocytes. However, it was found in the vicinity of matrix vesicles at the initial loci of calcification. Osteocalcin was never detected in the cartilage of warfarin-treated rats. Our results provide ultrastructural immunocytological evidence for the localization of endogenous osteocalcin in osteoblasts, the presence of osteocalcin in bone matrix and a direct gradient between the presence of osteocalcin and the calcification process. Osteocalcin is absent from cartilage, except possibly close to calcifying matrix vesicles. Warfarin inhibits the formation of osteocalcin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01625731
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  • 3
    Electronic Resource
    Electronic Resource
    Prolactin (PRL)-coding mRNA in Typhlonectes compressicaudus, a viviparous gymnophionan amphibian: an in situ hybridization study (1995)
    Springer
    Cell & tissue research 280 (1995), S. 133-138 
    Details
    ISSN: 1432-0878
    Keywords: Reproductive system, female ; Reproductive system, male ; Pituitary gland, pars anterior ; Prolactin cells ; Hybridization, in situ ; Typhonectes compressicaudus (Gymnophiona)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A study of prolactin-mRNA expression in the hypophysis of male and female Typhlonectes compressicaudus was carried out, using a quantitative in situ hybridization technique. In both males and females, an increase in the number of cells showing prolactin-like expression was observed at the beginning of the dry season. In the middle of the season, this number decreased. In pregnant females, mRNA expression remained constant during the first stages of gestation but, in the final stages, when embryos reached their maximum development, mRNA expression increased. In males and in sexually inactive females, prolactin-mRNA expression was the same throughout the year. We conclude that the activity of cells expressing prolactin could be related to hydromineral regulation and, in females, to the gestation process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304518
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Prolactin (PRL)-coding mRNA in Typhlonectes compressicaudus, a viviparous gymnophionan amphibian: an in situ hybridization study (1995)
    Springer
    Cell & tissue research 280 (1995), S. 133-138 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Reproductive system ; female ; Reproductive system ; male ; Pituitary gland ; pars anterior ; Prolactin cells ; Hybridization ; in situ ; Typhonectes compressicaudus (Gymnophiona)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. A study of prolactin-mRNA expression in the hypophysis of male and female Typhlonectes compressicaudus was carried out, using a quantitative in situ hybridization technique. In both males and females, an increase in the number of cells showing prolactin-like expression was observed at the beginning of the dry season. In the middle of the season, this number decreased. In pregnant females, mRNA expression remained constant during the first stages of gestation but, in the final stages, when embryos reached their maximum development, mRNA expression increased. In males and in sexually inactive females, prolactin-mRNA expression was the same throughout the year. We conclude that the activity of cells expressing prolactin could be related to hydromineral regulation and, in females, to the gestation process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304518
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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