ISSN:
1573-8221
Keywords:
tuberculosis
;
pathogen
;
nested-PCR
;
specificity
;
efficiency
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A test-system based on amplification of IS 986 fragment (nested-PCR) was developed for the detection ofMycobacterium tuberculosis andM. bovis in different biological samples. We constructed external primers and selected appropriate amplification parameters (annealing temperatures for stages I and II, the number of cycles for each amplification stage, components of the amplification mixture, and pretreatment conditions for different biological samples). The developed parameters make the detection of mycobacteria more efficient and less expensive compared to commercial Cobas Amplicor system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02434892
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