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  • 1
    Electronic Resource
    Electronic Resource
    Golgi Apparatus and Plant Secretion (1966)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 17 (1966), S. 27-46 
    Details
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.pp.17.060166.000331
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  • 2
    Electronic Resource
    Electronic Resource
    Membrane Biogenesis (1975)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 26 (1975), S. 441-481 
    Details
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.pp.26.060175.002301
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  • 3
    Electronic Resource
    Electronic Resource
    The absence of gangliosides in a higher plant (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 1433-1434 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We report attempts to isolate and purify sialic acid-containing glycolipids (gangliosides) from etiolated hypocotyls of soybean (Glycine max) using methods developed for rat liver. The maximum amounts of ganglioside sialic acid present was found to be less than. 0.021 nmole/g fresh weight or less than 1:100,000 the amounts present in rat liver. We conclude that this tissue lacks gangliosides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01932332
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  • 4
    Electronic Resource
    Electronic Resource
    Some unusual staining properties of tannic acid in plants (1987)
    Springer
    Histochemistry and cell biology 88 (1987), S. 17-22 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Maize root tips were fixed in glutaraldehyde fixatives containing tannic acid and then processed for electron microscopy. Under these conditions, tannic acid selectively stained the contents of the Golgi apparatus secretory vesicles of some outer root cap cells, the cell walls of all cells, and substances in, and adjacent to, intercellular connections of mature primary walls and of secondary walls. Intercellular connections of the young primary walls were not stained. Plasma membranes, and substances associated with the outer leaflets of the plasma membranes, were also stained. Tannic acid-positive material was associated with the cell plate vesicles of forming walls but very little, or none, was associated with the Golgi apparatus vesicles of dividing cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490161
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  • 5
    Electronic Resource
    Electronic Resource
    Tubular and filamentous structures in pollen tubes: Possible involvement as guide elements in protoplasmic streaming and vectorial migration of secretory vesicles (1972)
    Springer
    Planta 105 (1972), S. 317-341 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An ultrastructural study of the pollen tubes of Lilium and Clivia has demonstrated three different classes of longitudinal structures which could influence patterns of protoplasmic streaming and/or serve as “guide elements” in the vectorial migration of secretory vesicles: (a), cortical and noncortical microtubules; (b), microfilaments; and (c), subcortical tubules and cisternae of the endoplasmic reticulum (“subsurface cisternae”). Morphological details of these structures are described. Colchicine concentrations which lead to the complete disappearance of the microtubules affect neither germination of the pollen nor cytoplasmic streaming and tip growth of the elongating pollen tubes. Tip growth is initially uninhibited by cycloheximide, and cytoplasmic streaming is insensitive to this inhibitor. However, both of these processes are sensitive to cytochalasin B and vinblastine. Our results suggest that neither microtubules nor subsurface cisternae are essential for cytoplasmic streaming and directional secretion of cell surface materials in the pollen tube but would be consistent with an involvement of microfilamentous structures in these processes. Additionally, the possible importance of the lateral cross-link elements interconnecting all three types of structures is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386769
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  • 6
    Electronic Resource
    Electronic Resource
    Activity of Triclopyr Herbicide Enhanced by Combination with Cobalt Chloride or Ammonium Nitrate (1998)
    Springer
    Journal of plant growth regulation 17 (1998), S. 125-129 
    Details
    ISSN: 1435-8107
    Keywords: Key Words. Herbicide—Triclopyr—2,4-Dichlorophenoxyacetic acid—Cobalt—Ammonium nitrate—Synergism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Cobalt chloride at a rate of 16 oz/acre when combined with the auxin herbicide triclopyr at 1 oz/acre synergistically enhanced the activity of the herbicide. A similar response was elicited by the addition of ammonium nitrate. Mg2+ or Mn2+ was antagonistic. The enhancement of activity by Co2+ or NH+ 4 appeared not to be the result of a simple enhancement of triclopyr uptake by the cations. Triclopyr uptake, as determined by gas chromatography/microwave-induced plasma/chemical reaction interface mass spectroscopy, was unaffected by the cation additions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00007025
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  • 7
    Electronic Resource
    Electronic Resource
    Transformation with SV40 virus prevents retinoic acid inhibition of plasma membrane NADH diferric transferrin reductase in rat liver cells (1988)
    Springer
    Journal of bioenergetics and biomembranes 20 (1988), S. 383-391 
    Details
    ISSN: 1573-6881
    Keywords: Transplasma membrane electron transport ; plasma membranes ; retinoic acid ; cell differentiation ; SV40 transformation ; adriamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40°C. When the cells are in the transformed state (grown at the permissive 33°C temperature), retinoic acid does not inhibit the diferric transferrin reduction. Inhibition of activity of nontransformed cells is specific for retinoic acid with only slight inhibition by retinol and retinyl acetate at higher concentrations. Isolated rat liver plasma membrane NADH diferric transferrin reductase is also inhibited by retinoic acid. The effect of transformation with SV40 virus to decrease susceptibility to retinoic acid inhibition stands in contrast to much greater adriamycin inhibition of diferric transferrin reduction in the transformed cells than in nontransformed cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00769639
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and ultrastructural identification of membranes from the fungusGilbertella persicaria (1982)
    Springer
    Protoplasma 111 (1982), S. 87-106 
    Details
    ISSN: 1615-6102
    Keywords: Fungi ; Gilbertella persicaria ; Membranes ; Mitochondria ; Organelle isolation ; Plasma membrane ; Ultrastructure ; Vacuoles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Methods are described for isolating and identifying subcellular membranes from walled hyphae ofGilbertella persicaria. Differences in thickness and symmetry of membranes and in contents of vesicles were used to distinguish different types of membranes. Mitochondria, vacuoles, plasma membrane, and vesicles with attached ribosomes from homogenized germlings equilibrated at the 1.2/1.4 M interface in discontinuous sucrose gradients. Accelerated flotation in centrifuged Ficol-sucrose gradients resulted in the additional separation of the mixed membranes into three fractions: one contained predominantly intact mitochondria, another was composed of vacuoles and vesicles coated with ribosomes, and a third was enriched in plasma membranes. Based upon morphometric analysis, these fractions contained 92% mitochondria, 53% vacuoles, and 89% plasma membranes, respectively. The source of vesicles coated with ribosomes was investigated since rapidly growing hyphae ofG. persicaria contained little rough endoplasmic reticulum as compared with other classes of membranes. Reconstruction from electron micrographs of mitochondrial fragmentation and vesiculation suggested that most of the ribosome-coated vesicles originated from disrupted mitochondria rather than from rough endoplasmic reticulum. The study demonstrates the utility of ultrastructural markers to identify membranesin vitro independent of, or as an adjunct to, cytochemical and biochemical markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01282067
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  • 9
    Electronic Resource
    Electronic Resource
    Monensin affects the trans half ofEuglena dictyosomes (1983)
    Springer
    Protoplasma 114 (1983), S. 119-124 
    Details
    ISSN: 1615-6102
    Keywords: Monensin ; Ionophore ; Euglena ; Golgi apparatus ; Dictyosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Euglena gracilis was treated in 10-5 M monensin for various times from 2 minutes to 24 hours, and then processed for electron microscopy by fixation in glutaraldehyde and osmium tetroxide or potassium permanganate. Monensin affected the mature (trans) half of the cisternae but not the forming (cis) half of the cisternae. After glutaraldehyde and osmium tetroxide fixation, the affected cisternae appeared swollen, whereas after potassium permanganate fixation, the affected cisternae were distorted but not swollen. The monensin effect was first noticeable after 5 minutes of treatment and the maximum effect was observed after only 10 minutes of treatment. No additional monensin effects were observed up to 24 hours of treatment; however, by 24 hours there was variability in dictyosome form and some dictyosomes appeared relatively normal. The first noticeable effects at the 5 minute treatment time involved either the most mature (trans) cisterna or cisternae in the middle of the stack. Thus, inEuglena, the region of the Golgi apparatus that responds to monensin by cisternal dilation is restricted to the mature (trans) half of the dictyosomes, with the initial response given by specific cisternae in the stack.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279875
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  • 10
    Electronic Resource
    Electronic Resource
    NADPH-cytochrome c reductase and succinate-INT reductase of soybean membranes: An evaluation of two enzyme markers (1985)
    Springer
    Protoplasma 128 (1985), S. 14-21 
    Details
    ISSN: 1615-6102
    Keywords: Endoplasmic reticulum ; Marker enzymes ; Mitochondria ; Soybean (Glycine max)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This communication confirms the validity of two marker enzymes, NADH-cytochrome c reductase and succinate dehydrogenase (as succinate-INT reductase). for quantitation of the content of endoplasmic reticulum and mitochondria of plant cell fractions. Membrane fractions enriched to varying degrees in rough endoplasmic reticulum and mitochondria as well as reference fractions containing smooth membranes and dictyosomes were prepared from homogenates of etiolated soybean (Glycine max L.) hypocotyls. The NADPH-cytochrome c reductase activities of the fractions correlated with the content of rough endoplasmic reticulum as determined by morphometry; succinate-INT reductase activity correlated with the content of mitochondria. The absolute specific activity for pure endoplasmic reticulum was calculated from the regression analysis to be 17.8 Μmoles cytochrome c reduced hr−1 mg−1 protein and for mitochondria was determined to be 37.1 Μmoles INT reduced hr−1 mg−1 protein. These values permit estimation of endoplasmic reticulum and mitochondrial contamination of soybean fractions in the absence of pure reference fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273230
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