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  • 1
    Electronic Resource
    Electronic Resource
    EXPRESSION OF HUMAN CD4 BY TWO HUMAN-MOUSE INTERLINEAGE HYBRIDS (1988)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 15 (1988), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Two hybrid cell lines expressing human CD4 were prepared by fusing human B-lymphoid cells with the mouse T-lymphoma BW5147. Hybrid TF42 was derived from a human B-lymphoblastoid line and TF53.1 from a human B-ALL. Variants of these hybrids expressing or lacking CD4 were isolated by sorting cells stained with the monoclonal antibody (mAb) OKT4 on a fluorescence-activated cell sorter (FACS). Cytogenetic, isoenzyme and DNA analysis confirmed the presence of human chromosome 12 in the CD4+ hybrids, and revealed that CD4 expression by TF42 was associated with multiple copies of this chromosome. Of seventy mAb recognizing human T-cell antigens screened on the CD4+ and CD4− variants of the two hybrids, only mAb recognizing CD4 and Leu 8 reacted with the CD4+ cells. These hybrids should be useful in the preparation, screening and analysis of anti-CD4 monoclonal antibodies, and in studies of CD4 epitopes recognized by HIV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1988.tb00422.x
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  • 2
    Electronic Resource
    Electronic Resource
    Gene mapping and physical arrangements of human chromatin in transformed, hybrid cells: Fluorescent and autoradiographic in situ hybridization compared (1986)
    Springer
    Somatic cell and molecular genetics 12 (1986), S. 313-324 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We compare a fluorescent in situ hybridization technique, usingN-acetoxy-2-acetylaminofluorene (N-ACO-AAF) modified DNA adducts, with3H-labeled DNA in situ hybridization for (1) visualizing human transgenomes in HRAS1-selected, chromosome-mediated gene transfer (CMGT), and (2) mapping chromosomal SV40 in an SV40-transformed, human-mouse hybrid cell line. We demonstrate that individual HRAS1-CMGTs may contain multiple fragments of human chromatin. We deduce that the CMGT process can involve interstitial loss of mouse chromatin. We conclude that the N-ACO-AAF technique gives finer resolution than3H-labeled in situ hybridization. However,3H-labeling is more sensitive and has allowed us to sublocalize SV40 in Cl21 to the region 7q31–35.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01570725
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    Paper (German National Licenses)
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