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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Chromatography B: Biomedical Sciences and Applications 614 (1993), S. 164-168 
    ISSN: 0378-4347
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 12 (1998), S. 331-334 
    ISSN: 1432-2218
    Keywords: Key words: Laparoscopic surgery — Hepatic surgery — Liver — Four-hands technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: An effort was made to evaluate the indications, safety, and therapeutic efficacy of laparoscopic liver surgery. Methods: Between 1989 and 1996, 28 patients, 23 to 88 years old were operated upon laparoscopically. Pathology consisted of simple cyst (ten), polycystic liver disease (seven), hydatid cyst (three, two of them calcified), abscess (one), focal nodular hyperplasia (six), and metastatic breast cancer (one). Results: Operations included 17 fenestrations, three pericystectomies, and eight resections (two lateral lobes). Operative time was 45 to 525 min with only four cases longer than 4 h. There was a 21% morbidity rate. There were no mortalities. Follow-up was 1–67 months with one asymptomatic recurrence. Conclusions: Laparoscopic hepatic surgery can be performed safely with good results by surgeons with hepatic and laparoscopic experience when careful selection criteria are followed. We advocate the ``four-hands technique'' for simultaneous dissection and control of bleeding and bile ducts during resections.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 12 (1998), S. 1051-1054 
    ISSN: 1432-2218
    Keywords: Key words: Surgery-laparoscopic — Vagotomy — Duodenal ulcer — Harmonic shears
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Results from classic highly selective vagotomy (HSV) are technique dependent because an incomplete operation will result in early recurrence of duodenal ulcer. Few reports describe laparoscopic completion of the procedure. All techniques use clips for division of neurovascular branches, making the laparoscopic approach tedious and thus the results, uncertain. Methods: Ten patients with intractable duodenal ulcer and negative Helicobacter pylori status underwent an extended HSV. All procedures were performed laparoscopically using a new surgical tool, the harmonic shears. Results: All procedures were completed laparoscopically and took approximately 1 h. There were no deaths and no postoperative complications. Patients were discharged the next day. Follow-up endoscopy at 2 months showed healing of duodenal ulcer in all cases, and postoperative acid secretion studies demonstrated a decrease in basal acid output (BAO) by 74% (8.2 meq/h to 2.16 meq/h) and maximal acid output (MAO) by pentagastrin stimulation by 79.2% (40 to 8.32). Conclusions: Harmonic shears expedite laparoscopic HSV. The operation can be taught safely, yields good results in early follow-up, and represents an acceptable option in patients with intractable duodenal ulcers who are H. pylori negative.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    World journal of surgery 17 (1993), S. 34-39 
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Deux procédés sous coelioscopie peuvent Être utilisés pour le traitement de l'ulcère duodénal chronique: la vagotomie tronculaire bilatérale avec pyloroplastie par dilatation endoscopique au ballonnet et la vagotomie tronculaire postérieure associée à la séromyotomie antérieure. Si le premier est facile à réaliser avec des résultats valables, le second doit Être préféré car il respecte la physiologie de l'estomac. Trente-six patients, opérés en 18 mois et ayant des résultats comparables à notre expérience en chirurgie traditionnelle font l'objet de ce travail. L'indication de la chirurgie a été une maladie ulcéreuse chronique du duodénum résistante au traitement médical optimal. Aucune morbidité ou mortalité périopératoire n'a été enrégistrée. Il n'y a pas eu de complication post-opératoire précoce. Le rÔle de la vagotomie par coelioscopie dans le traitement de l'ulcère duodénal chronique ne paraÎt pas clair. La méthode de vagotomie coelioscopique nécessite une évaluation dans les centres rompus à ce type de chirurgie avant de préconiser une application clinique étendue. Des études prospectives multicentriques avec un suivi à long-terme sont nécessaires pour évaluer les résultats de cette technique dans la maladie ulcéreuse.
    Abstract: Resumen Se describen dos procedimientos laparoscópicos para el tratamiento de la Úlcera duodenal crónica: vagotomia troncular bilateral con dilatación pilórica por medio de balón y vagotomía troncular posterior con seromiotomía anterior de la curvatura menor. En tanto que el primer procedimiento es de sencilla realización y es fácilmente reproducible, el segundo es el preferido porque respeta la fisiología del estómago. Se operaron 36 pacientes en un periodo de 18 meses con buenos resultados, enteramente comparables con la experiencia de la cirugía abierta. Las indicaciones para cirugía fueron la presencia de Úlceras duodenales crónicas intratables, o sea aquellas resistentes a terapia médica óptima. No se presentó morbilidad ni mortalidad perioperatorias y no se han encontrado Úlceras recurrentes en el seguimiento postoperatorio temprano. AÚn no está claro cual es el papel real de la cirugía laparoscópica en el arsenal terapéutico de la Úlcera duodenal. El método de la vagotomía laparoscópica requiere una rigurosa valoración experimental en centras especializados antes de una generalizada aplicación clínica. Se requieren futuros estudios prospectivos multicéntricos con seguimientos a largo plazo para determinar los resultados de esta terapia innovadora en la enfermedad ácido-péptica.
    Notes: Abstract Two laparoscopic procedures for treatment of chronic duodenal ulcer are described: bilateral truncal vagotomy with balloon pyloric dilatation and posterior truncal vagotomy with anterior lesser curve seromyotomy. The first procedure is simple to perform and easily reproducible, but the latter is preferred because it respects the physiology of the stomach. Thirty-six patients were operated on over a period of 18 months with good results comparable to those with open surgery. The indications for surgery were intractable chronic duodenal ulcers resistant to optimal medical therapy. There was no perioperative morbidity or mortality, and recurrent ulcers have not been demonstrated during early postoperative follow-up. The proper role of laparoscopic surgery in the arsenal of treatment of duodenal ulcers is unclear. The method of laparoscopic vagotomy requires rigorous experimental evaluation in specialized centers before widespread clinical application. Future multicentric prospective studies with long-term follow-up are necessary to assess the results of this innovative therapy of acid-peptic disease.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2277
    Keywords: Key words Normothermic liver ischemia ; Apoptosis ; Caspases ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Normothermic ischemia and reperfusion of the liver results in microcirculatory failure followed by necrosis and cell death. Recently, another type of cell death, apoptosis or programmed cell death, was found to be activated during the early phase of reperfusion after liver ischemia. Caspases are cysteine proteinases specifically involved in the initiation and execution phases of apoptosis. The aim of this study was to demonstrate that inhibition of apoptosis by a specific inhibitor of caspases might protect the liver against ischemia/reperfusion injury. Rats were divided into three groups: group 1, control, PBS administration; group 2, Z-Asp-cmk (Z-Asp-2,6-dichlorobenzoyl-oxymethylketone) treatment; group 3, sham-operated control animals. Z-Asp-cmk (0.5 mg Z-Asp-cmk dissolved in 300 μl PBS solution containing 1 % DMSO) was injected intravenously, 2 min prior to induction of 120 min ischemia. Survival rates were compared and serum activities of aspartate aminotransferases and alanine aminotransferases were assessed in the blood collected from the suprahepatic vena cava. Histology of the liver was assessed 6 h after the end of ischemia. Apoptosis was detected by the terminal deoxynucleotidyl transferase-mediated dUTP-FITC nick end-labeling method (TUNEL method) and by electrophoresis for analysis of DNA fragmentation. Caspase activity was determined by measuring hydrolysis of the CPP32-like substrate Ac-DEVD-pNA and absorption of paranitroaniline. Z-Asp-cmk treatment significantly increased 7-day survival (95 %) compared with that in nontreated rats (30 %, P 〈 0.001). Serum activities of aminotransferases and the extent of liver congestion and necrosis were significantly (P 〈 0.001) decreased after treatment with Z-Asp-cmk. TUNEL-positive cells were detected 3–6 h after reperfusion in the control group. In Z-Asp-cmk pretreated rats, a dramatic decrease in the number of TUNEL-positive cells was observed. Analysis of DNA fragmentation of freshly isolated hepatocytes confirmed these results. Caspase activity was increased 3–6 h after reperfusion in the control group, but significantly (P 〈 0.001) decreased after treatment with Z-Asp-cmk. These findings demonstrate that liver injury following ischemia and reperfusion can be prevented by inhibition of caspases. Caspase inhibitors may have important implications for therapy in liver disease and after liver transplantation.
    Type of Medium: Electronic Resource
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