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  • 1
    Electronic Resource
    Electronic Resource
    An immunocytochemical comparison of cytoskeletal proteins in aluminum-induced and Alzheimer-type neurofibrillary tangles (1986)
    Springer
    Acta neuropathologica 70 (1986), S. 243-248 
    Details
    ISSN: 1432-0533
    Keywords: Aluminum ; Rabbit ; Immunocytochemistry ; Alzheimer's disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Exposure of the central nervous system (CNS) of rabbits to aluminum salts produces a progressive encephalopathy. Examination of CNS strucres discloses widespread perikaryal neurofibrillary tangle (NFTs) formation. The aluminum-induced NFTs consist of collections of normal neurofilaments, and differ ultrastructurally and in their solubility characteristics from Alzheimer-type NFTs, the latter being composed of largely insoluble paired helical filaments. The present study compares NFTs found in the rabbit to those of Alzheimer's disease, using monoclonal antibodies (SMI 31, SMI 32) that recognize phosphorylated and non-phosphorylated determinants respectively in normal neurofilaments, and an antiserum raised against purified microtubules. Paraffin-embedded sections were stained by the avidin-biotin immunocytochemical method. Intense staining of aluminum-induced NFTs was found after processing with SMI 31 and SMI 32, while no staining of non-tangled perikarya of control rabbits or of Alzheimer-type NFTs was seen. Antimicrotubule antiserum gave weak, nonfocal staining in the aluminum-treated and control rabbits, while Alzheimer-type NFTs were stained intensely. These results show that phosphorylated and non-phosphorylated neurofilaments accumulate in aluminum-induced NFTs, thus complementing the previously demonstrated specific slowing of the axonal transport of neurofilaments in aluminum intoxication. Further, they suggest that the presence of microtubular proteins may be necessary for altered neurofilaments to take on a paired helical configuration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686078
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  • 2
    Electronic Resource
    Electronic Resource
    Gliogenesis in organotypic tissue culture of the spinal cord of the embryonic mouse. I. Immunocytochemical and ultrastructural studies (1986)
    Springer
    Journal of neurocytology 15 (1986), S. 273-290 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The technique of organotypic tissue culture offers an opportunity to observein vitro complex interactions among glial cells and neurons, leading to the formation of myelin. In the present and accompanying work a combined ultrastructural, immunocytochemical and autoradiographic approach was used in a detailed study of the process of gliogenesis. Using immunocytochemical and ultrastructural criteria, differentiation along the oligodendroglia cell line is seen to be initiated a few days later than along the astroglial line. The sequence and timing of oligodendroglial differentiation both ultrastructurally and chemically follow those describedin vivo. Formation of myelin has been demonstrated only by oligodendrocytes in which there is continuity between the perikaryal plasmalemma and myelin membranes. Oligodendroglial maturation culminated with the formation of light, medium and dark oligodendrocytes. The periodic acid Schiff-positive, glial fibrillary acidic protein (GFAP)-negative processes of radial glial cells at explantation become GFAP-positive within 3 days, as describedin vivo. Many of the astrocytes appear to have been derived from radial glial cells. Large numbers of dark glial cells, similar to the so-called ‘intermediate glial cells’, were seen. These were found to be astrocytes whose appearance probably reflected reaction to explantation-induced injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01611431
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Gliogenesis in organotypic tissue culture of the spinal cord of the embryonic mouse. II. Autoradiographic studies (1986)
    Springer
    Journal of neurocytology 15 (1986), S. 291-302 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Organotypic cultures of the spinal cord of the embryonic mouse were subjected to pulses of tritiated thymidine at various times between explantation and 42 daysin vitro (DIV). Autoradiography was performed both on cultures fixed immediately at the end of the pulse and on cultures maintained in radioactive-free medium for various periods after the pulse. Quantitative light autoradiographic studies showed a single peak of glial cell proliferation at 9 DIV equivalent to that demonstratedin vivo. The growth rate of glial cells (related to time in culture) decreased along an exponential decay type curve. All these observations were statistically significant when tested against the corresponding null hypothesis. Ultrastructural autoradiography shows that at early stages of the culture, radial glial cells and immature glial cells divided and eventually gave rise to astrocytes and oligodendrocytes. During the period of maximal cell proliferation, tritiated thymidine was incorporated by differentiated astrocytes and ultrastructurally recognizable immature oligodendrocytes. Oligodendrocytes did not divide beyond the stage of active oligodendrocytes (the cells initiating myelination). They were capable of producing dark oligodendrocytes within a week following the last division. These observations emphasize the similarity of the proliferation during development in organotypic culture to thatin vivo, modified by the trauma of explantation and the culture conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01611432
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    Paper (German National Licenses)
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