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  • 1
    ISSN: 0264-410X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Infection 17 (1989), S. 250-251 
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-7284
    Keywords: Flemish population ; Hepatitis A (HAV) ; Hepatitis B (HBV) ; Hepatitis C (HCV) ; Prevalence study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Viral hepatitis is a serious health problem throughout the world. No recent prevalence data on hepatitis A, B and C were available for the population in Flanders, Belgium. For this reason, a sero-epidemiological study was undertaken in 1993–1994 in a sample of the general population. The purpose of this study was to obtain a clear picture of the prevalence of hepatitis A, B and C. Between April 1993 and February 1994, 4,058 blood samples were drawn and collected in 10 hospitals in Flanders. The study group was representative for the Flemish population. For hepatitis A a seroprevalence of 55.1% was found. In the non-Belgian residents the HAV prevalence was significantly higher than in Belgians (62% versus 52%; χ2 = 8.05; p = 0.005). For hepatitis B, 9.9% of the study group showed serological evidence of hepatitis B markers: 6.9% of the participants was positive for anti-HBs/anti-HBc, 0.7% appeared to be HBsAg positive and 3.5% was solely anti-HBs positive. The prevalence of HBV markers in Belgians was 6.9%, significantly lower compared to the 13.4% among non-Belgians (χ2 = 14.05; p = 0.00018). 4055 serum samples were analysed for hepatitis C serology by second generation anti-HCV tests. Anti-HCV was detected in 0.87% of the serum samples. No statistically significant difference was found in HCV prevalence between Belgians and non-Belgians. Results of this study should help policy makers in their decisions on the most appropriate hepatitis A and B vaccination strategy and on the most effective prevention strategy for hepatitis C.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0584
    Keywords: Key words Platelet procoagulant activity ; Platelet concentrate ; Mean platelet volume.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Platelets stored as concentrates are gradually activated (storage lesion), a process associated with changes in the expression of platelet procoagulant activity (PPCA). The aim of the present study was to evaluate the evolution of PPCA and the mean platelet volume (MPV) of stored platelets prepared according to the platelet-rich method (PRM) and the buffy coat method (BCM). Using the platelet factor 3 availability clotting test (PF3AT) on appropriately diluted concentrate samples, we found a decrease in PPCA expression of remnant platelets as a function of storage time (0.025〈p〈0.01 between day 1 and 7) in PRM-derived but not in BCM-derived platelet concentrates. Using the PF3AT reduction test we found a more important clotting time reduction in samples obtained from BCM than in samples obtained from PRM platelet concentrates, suggesting a higher PPCA expression of BCM platelets, not significant after 1 day but highly significant after 3 days (p〈0.0005) and after 7 days (p〈0.0005) of storage, as compared with PRM platelets. For both PRM and BCM concentrates there were no significant MPV changes as a function of storage time, but at any storage day the MPV of BCM concentrates was significantly higher (p〈0.0005) than the MPV of PRM concentrates. We conclude that the decrease of PPCA expression in PRM-derived concentrates as a function of storage time is in agreement with the gradual decrease of the platelet activation status in PRM concentrates during storage. There are probably several factors or variables causing platelets of BCM concentrates to express higher PPCA than those of PRM concentrates. Higher PPCA expression in BCM concentrates may be explained by an intrinsic platelet property, such as a difference in MPV between the two kinds of concentrates, or it may be related to an extrinsic factor such as different storage media, e.g., undiluted autologous plasma in PRM concentrates versus Plasmalyte A-diluted autologous plasma in BCM concentrates. Whether the difference in PPCA expression of remnant platelets in PRM and BCM concentrates is just an in vitro laboratory finding or may have consequences for the therapeutic efficiency of the concentrates is an interesting, still unresolved question.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1437-1596
    Keywords: Key words DNA polymorphism ; Short tandem repeats (STRs) ; Paternity testing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract In order to apply a set of nine STR loci in parentage testing, we performed a population genetic study on a sample of the Flemish population. Genotypes for HUMHPRTB, HUMFABP, HUMCD4, HUMCSF1PO, HUMTH01, HUMPLA2A, HUMPLA2A1, HUMF13A01, HUMCYAR04 and HUMLIPOL were determined using three triplex PCR reactions and silver staining. Allele frequencies showed no deviation from Hardy-Weinberg equilibrium. The frequency distribution agreed well with other Caucasian populations but three intermediate fragments, not previously found in Caucasians, were observed. We then resolved a series of 151 parentage disputes of which 103 were exclusions. In six cases, evidence for exclusion was obtained by only one informative STR locus out of eight for male children or out of nine for female children. These exclusions were confirmed with additional polymorphic markers. In one case of inclusion, a paternal allele expanded with one repeat unit of HUMHPRTB. This observation illustrates that STRs do not differ from other genetic systems in the fact that more than one excluding locus is required before exclusion is demonstrated.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Infection 18 (1990), S. 364-366 
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Epidemiologische Daten aus verschiedenen Ländern lassen eine eindeutige Abhängigkeit der Prävalenz von Hepatitis A Virus-Antikörpern (anti-HAV) in verschiedenen Bevölkerungsgruppen von geographischen Gegebenheiten und der Altersverteilung der untersuchten Gruppe erkennen. In Südeuropa finden sich anti-HA-Antikörper in allen Gruppen relativ häufig, in Nordeuropa nur bei der älteren Bevölkerung. Prävalenzdaten, die für 1979 und 1989 bestimmt wurden, zeigten altersbezogene Unterschiede in der anti-HAV-Antikörper-Prävalenz, die Raten an positiven Befunden waren in allen Altersgruppen 1989 geringer als 1979. Im Jahre 1979 waren etwa 50% der 25- bis 30jährigen anti-HAV positiv, was sich auch in einer anderen belgischen Studie bestätigte, die 1979 durchgeführt wurde. 1989 wurde nur bei den 35- bis 40jährigen eine Rate von 50% anti-HAV-Positiven gefunden. Eine Abnahme der anti-HAV-Antikörper-Prävalenz mit höherem sozio-ökonomischen Status ließ sich auch in der vorliegenden Studie erkennen. Bedeutsam wird dies in Verbindung mit den hygienischen Verhältnissen dann, wenn die meisten Infektionen auftreten, das heißt vor dem 20. Lebensjahr. Die Vergleichsuntersuchungen der Prävalenzdaten 1979 und 1989 lassen annehmen, daß sich die Zahl der gegenüber HAV empfänglichen Erwachsenen erhöht hat. Dies ist im Hinblick auf die in weiten Teilen der Bevölkerung eingetretene Änderung der Reisegewohnheiten von Bedeutung.
    Notes: Summary Epidemiological data from various countries show that the frequency of Hepatitis A virus antibodies (anti-HAV) in different population groups is largely dependent on the geographical and age distribution of the population surveyed. As regards Europe anti-HAV antibodies are generally frequent in all groups in Southern Europe while in Northern Europe these antibodies are common in older people only. The prevalence data collected in 1979 and in 1989 show that the anti-HAV antibodies rate is a function of age, but the rates for all age groups were lower in 1989 compared to 1979. In 1979, at an age between 25 and 30, some 50% of the population was anti-HAV antibody positive. The same results were obtained in another Belgian study conducted in 1979. In 1989 50% positivity was only reached at an age between 35 and 40 years. The present study confirms that anti-HAV antibody prevalence decreases with higher socioeconomic status. The higher rate relative to age is associated with socioeconomic and hygienic living conditions at the time when most infections occur, i. e. before the age of 20. It can be concluded by comparing the 1979 and 1989 results that the number of adults susceptible to HAV infections has increased. This fact drew attention in view of the strongly altered travelling pattern of fairly large sections of the population.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1437-1596
    Keywords: Key words Short tandem repeat (STR) ; Mutation ; DNA analysis ; Alleles ; Paternity testing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract During routine paternity testing a mutation of a paternal allele at the HPRTB locus was observed. The opportunity was taken to analyse this mutation at a molecular level. The repeat sequence is flanked by an imperfect repeat sequence and this region could be involved in the mutation mechanism. For this reason, we also examined the structure of “intermediate” alleles. Sequencing confirmed the insertion of a perfect repeat motif and revealed a deletion of a dinucleotide some 50 nucleotides downstream from the repeat sequence for the intermediate alleles. It is likely that these intermediate alleles are rare biallelic deletion polymorphisms and are probably not involved in the mutation or variation mechanism of this locus.
    Type of Medium: Electronic Resource
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