ISSN:
1432-1432
Keywords:
Key words:β-Globin —δ-Globin — Embryonic globin —ε-Globin —δ-Globin — Gene cluster — Hemoglobin — Multigene family
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. The β-globin gene cluster of Wistar rat was extensively cloned and the embryonic genes were mapped and sequenced. Four overlapping λ Dash recombinant clones cover about 31 kb and contain four nonadult β-globin genes, 5′–ε1–γ1–γ2–ψγ3–3′. The ε1 and γ2 are active genes, since their protein products were detected in the fetal stage of the rat (Iwahara et al., J Biochem 119:360–366, 1996). The γ1 locus might be a pseudogene, since the ATA box in the promoter region is mutated to GTA; however, no other defect is observed. The ψγ3 locus is a truncated pseudogene because a 19-base deletion, which causes a shift of the reading frame, is observed between the second nucleotide of the putative codon 68 and codon 76. A sequence comparison suggests that the ψγ3 might be produced by a gene conversion event of the proto-γ-globin gene set. Possible histories of the evolution of rat nonadult β-globin genes are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00006525
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