ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The strategies developed for gene delivery are generally classified into two categories ofviral and non-viral vectors. The limitation of viral vectors, which have problems including toxicity,immunogenicity and inflammatory response has led to the development of a novel, synthetic vectorsbased on non-viral vectors. Chitosan, one of non-viral vectors, has been a good candidate in genedelivery field. Moreover, galactosylated chitosan (GC) had the specific recognition of hepatocytesby galactose in the GC. Also, carbonate apatite increased the rate of DNA endocytosis and theefficiency of gene transfer. We describe here a new concept for improving cell specificity andtransfection efficiency by hybridization of carbonate apatite (CAp) with GC. The complexformation was confirmed by agarose gel electrophoresis. The complex optimized throughcontrolling calcium ion and charge ratio was evaluated on the cell specificity and transfectionefficiency
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.437.pdf
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