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Digitale Medien

The Gene for the Leu-M2 (Mac-120) Antigen is Located on Human Chromosome 11 (1987)

SUOMALAINEN-NEVANLINNA, H. A. ; SCHRÖDER, J. ; ANDERSSON, L. C.

Oxford, UK : Blackwell Publishing Ltd

Scandinavian journal of immunology 25 (1987), S. 0

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Details

ISSN: 1365-3083

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Leu-M2 (Mac-120) antigen is a cell-surface marker present on a subpopulation of human blood monocytes and platelets. Its expression on monocytes and macrophages is necessary for their ability to present antigen to T lymphocytes. Only Leu-M2+ cells display the human la-like determinant, HLA-DS, which seems important in antigen presentation and stimulation in the autologous MLR. Expression of the Leu-M2/Mac-120 antigen was studied by indirect immunofluorescence analysis and flow cytofluorometry in mouse-human somatic cell hybrids segregating human chromosomes. In the mouse-human hybrid clones, expression of the Leu-M2/Mac-120 antigen was dependent on the presence of human chromosome 11. This was verified by fluorescence-activated cell sorting of heterogeneous hybrid cell populations into antigenpositive and -negative fractions. The Leu-M2 antigen co-segregated with chromosome 11. The gene controlling the expression of the Leu-M2/Mac-120 antigen thus is assigned to human chromosome 11.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1365-3083.1987.tb02209.x

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Digitale Medien

Generation of a panel of somatic cell hybrids containing unselected fragments of human chromosome 10 by X-ray irradiation and cell fusion: Application to isolating theMEN2A region in hybrid cells (1990)

Goodfellow, P. J. ; Povey, S. ; Nevanlinna, H. A. ; [weitere]

Springer

Somatic cell and molecular genetics 16 (1990), S. 163-171

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ISSN: 1572-9931

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract We have used X-ray irradiation and cell fusion to generate somatic cell hybrids containing fragments of human chromosome 10. Our experiments were directed towards isolating the region of theMEN2A gene in hybrids and to use those as the source of DNA for cloning and mapping new markers from near theMEN2A locus. A number of hybrid clones containing human sequences that are tightly linked to theMEN2A gene were identified. Some 25% of our hybrids, however, proved to contain more than one human chromosome 10-derived fragment or showed evidence of deletions and/or rearrangements. A detailed analysis of the human content of X-ray irradiation hybrids is required to assess the integrity and number of human fragments retained. Despite retention of multiple human-derived fragments, these hybrids will prove useful as cloning and mapping resources.