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  • 1
    Electronic Resource
    Electronic Resource
    Homologous recombination partly restores the secretion defect of underglycosylated acid phosphatase in yeast (1997)
    Springer
    Current genetics 32 (1997), S. 190-196 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsPHO5 ; PHO3 ; Tandem repeats ; Intrachromosomal recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The majority of secreted acid phosphatase in Saccharomyces cerevisiae is encoded by the PHO5 gene. The secretion level of this acid phosphatase is directly determined by its level of glycosylation. Consequently, PHO5-11-encoded acid phosphatase which lacks 11 of 12 glycosylation sites is only poorly secreted. We have isolated and characterized both UV- and EMS-induced variants, which are partly able to restore the secretion of acid phosphatase. Our data indicate that the improved secretion is caused by mitotic intrachromosomal recombination between the PHO5-11 allele and the homologous tandemly repeated PHO3 sequences, resulting in the restoration of glycosylation sites in PHO5-11. Two different recombination mechanisms, unequal sister-chromatid exchange and sister-chromatid gene conversion, are responsible for these alterations of the PHO5-11 locus. Thus, recombination between mutant and wild-type sequences are able to restore the ability of mutant yeast cells to secrete acid phosphatase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050265
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  • 2
    Electronic Resource
    Electronic Resource
    When is the Male Plastome eliminated ? (1970)
    [s.l.] : Nature Publishing Group
    Nature 227 (1970), S. 1265-1266 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Table 1. ORIGIN AND PHENOTYPE OF HAPLOID PLANTLETS ARISING FROM ANTHER CULTURE OF N. tabdCUm VARIETY SAMSUN AND A MATERNALLY INHERITED PLASTOME MUTANT Shoot Plant phenotype, phenotype per cent white Green 0 (green) Variegated 0 (green) 30 30-50 50 50-60 50-80 No. of shoots used 6 1 1 2 1 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2271265a0
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  • 3
    Electronic Resource
    Electronic Resource
    A fungal minisatellite (1997)
    [s.l.] : Nature Publishing Group
    Nature 386 (1997), S. 771-771 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Several minisatellites, or variable number Oof tandem repeats, have been described in the nucleotide sequences of mammalian1, insect2, fish3 and bird4 genomes. We present here sequence data of a minisatellite found in the lager brewing yeast Saccharomyces carlsbergensis. We were surprised to detect ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/386771a0
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Studies on the biosynthesis of TMV (1970)
    Springer
    Molecular genetics and genomics 109 (1970), S. 246-256 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The replicative form and the replicative intermediate of TMV-RNA were isolated from synchronously infected tobacco leaves, labeled with H3-uridine for 1 hour. The replicative form is over 90% resistant to RNase and sediments slightly slower than the 16S ribosomal RNA. Sucrose gradient centrifugation of the thermally denatured replicative form revealed that it contains strands of the same size as single-stranded TMV-RNA. The replicative intermediate showed only partial resistance to RNase and heterogeneous sedimentation behavior in sucrose gradients. After mild RNase treatment the replicative intermediate sedimented homogeneously, and with an S value slightly lower than the replicative form.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267013
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  • 5
    Electronic Resource
    Electronic Resource
    Recombinational repair of diverged DNAs : a study of homoeologous chromosomes and mammalian YACs in yeast (1992)
    Springer
    Molecular genetics and genomics 234 (1992), S. 65-73 
    Details
    ISSN: 1617-4623
    Keywords: Recombinational repair ; Double-strand breaks ; YACs ; Divergent DNA ; Mismatch repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Recombinational repair is the means by which DNA double-strand breaks (DSBs) are repaired in yeast. DNA divergence between chromosomes was shown previously to inhibit repair in diploid G1 cells, resulting in chromosome loss at low nonlethal doses of ionizing radiation. Furthermore, 15–20% divergence prevents meiotic recombination between individual pairs of Saccharomyces cerevisiae and S. carlsbergensis chromosomes in an otherwise S. cerevisiae background. Based on analysis of the efficiency of DSB-induced chromosome loss and direct genetic detection of intragenic recombination, we conclude that limited DSB recombinational repair can occur between homoeologous chromosomes. There is no difference in loss between a repair-proficient Pms+ strain and a mismatch repair mutant, pms1. Since DSB recombinational repair is tolerant of diverged DNAs, this type of repair could lead to novel genes and altered chromosomes. The sensitivity to DSB-induced loss of 11 individual yeast artificial chromosomes (YACs) containing mouse or human (chromosome 21 or HeLa) DNA was determined. Recombinational repair between a pair of homologous HeLa YACs appears as efficient as that between homologous yeast chromosomes in that there is no loss at low radiation doses. Single YACs exhibited considerable variation in response, although the response for individual YACs was highly reproducible. Based on the results with the yeast homoeologous chromosomes, we propose that the potential exists for intra- YAC recombinational repair between diverged repeat DNA and that the extent of repair is dependent upon the amount of repeat DNA and the degree of divergence. The sensitivity of YACs containing mammalian DNA to ionizing radiation-induced loss may thus be an indicator of the extent of repeat DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272346
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    Paper (German National Licenses)
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