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  • 1
    Electronic Resource
    Electronic Resource
    Membrane-associated redox activities in Thermotoga neapolitana (1998)
    Springer
    Archives of microbiology 170 (1998), S. 297-303 
    Details
    ISSN: 1432-072X
    Keywords: Key words Hyperthermophile ; Hydrogenase ; Oxidoreductase ; Ferredoxin ; Membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Elemental sulfur reduction by the hyperthermophilic bacterium Thermotoga neapolitana provides an alternative to hydrogen evolution during fermentation. Electrons are transferred from reduced cofactors (ferredoxin and NADH) to sulfur by a series of unknown steps. One enzyme that may be involved is an NADH:methyl viologen oxidoreductase (NMOR), an activity that in other fermenting organisms is associated with NADH:ferredoxin oxidoreductase. We found that 83% of NMOR activity was contained in the pellet fraction of cell extracts subjected to ultracentrifugation. This pellet fraction, presumably containing cell membranes, was required for electron transfer to NAD+ from ferredoxin-dependent pyruvate oxidation. However, the NMOR activity in this fraction used neither Thermotoga nor clostridial ferredoxins as substrates. NMOR activity was also detected in aerobically prepared vesicles. By comparison with ATPase activities, NMOR was found primarily on the cytoplasmic face of these vesicles. During these studies, an extracytoplasmic hydrogenase activity was discovered. In contrast to the soluble hydrogenase, this hydrogenase activity was completely inhibited when intact cells were treated with cupric chloride and was present on the extracytoplasmic face of vescides. In contrast to a soluble hydrogenase reported in Thermotoga maritima, this activity was air-stable and was inhibited by low concentrations of nitrite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050645
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Recent advances in genetic analyses of hyperthermophilic Archaea and Bacteria (1997)
    Springer
    Archives of microbiology 168 (1997), S. 73-80 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsArchaea ; Hyperthermophile ; Thermophile ; Sulfolobus ; Pyrococcus ; Thermotoga ; Methanococcus ; jannaschii ; Vectors ; Genetic markers ; Thermostable ; proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hyperthermophilic Archaea and Bacteria are an extraordinarily important class of organisms for which genetic tools remain to be developed. Unique technological obstacles to this goal are posed by the thermophilic and, in some cases, strictly anaerobic nature of these organisms. However, recent advances in the cultivation of hyperthermophiles, in the discovery of genetic elements for vector development, and in the construction of genetic markers point toward the achievement of this goal in the near future. Transformation protocols have already been reported for Sulfolobus and Pyrococcus, and plasmid-mediated conjugation was recently found in Sulfolobus. Plasmids are available for Sulfolobus, Pyrococcus, and the bacterial hyperthermophile Thermotoga, and these provide the bases for vector construction in these hosts. A Desulfurococcus mobile intron may provide a novel means to introduce genes into a variety of archaeal hosts. With full genome sequences of several hyperthermophiles available soon, genetic tools will allow full exploitation of this information to study these organisms in depth and to utilize their unique properties in biotechnological applications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050472
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    Paper (German National Licenses)
    Fulltext
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