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  • 1
    Electronic Resource
    Electronic Resource
    The involvement of ubiquitin in vegetative desiccation tolerance (1999)
    Springer
    Plant molecular biology 41 (1999), S. 657-667 
    Details
    ISSN: 1573-5028
    Keywords: desiccation-tolerant ; repair mechanisms ; Sporobolus stapfianus ; Tortula ruralis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated a polyubiquitin cDNA from the modified desiccation-tolerant grass Sporobolus stapfianus. This cDNA, along with a commercially available polyclonal ubiquitin antibody, was used to characterize desiccation/rehydrated-associated changes in ubiquitin-mediated protein degradation in S. stapfianus and the fully desiccation-tolerant moss Tortula ruralis. Northern analysis demonstrated that in S. stapfianus leaves two ubiquitin transcripts, of ca. 1.4 and 1.2 kb, accumulated above control levels during drying and rehydration but were barely detectable in desiccated tissue. The peak in rehydration-associated transcript accumulation coincided with a depletion in ubiquitin monomer levels indicating an increase in protein degradation. Analysis of T. ruralis revealed three ubiquitin transcripts of ca. 1.9, 1.3 and 0.65 kb, with only the 1.3 kb transcript level varying in response to drying and rehydration and all transcripts being stable in dried tissue. Western analysis revealed that conjugated ubiquitin, indicative of proteins targeted for removal, was evident in all samples of Sporobolus but detectable only in slow-drying Tortula which also displayed reduced levels of ubiquitin monomer. These results demonstrate that desiccated T. ruralis gametophyte possesses stable ubiquitin transcripts which can be translated upon rehydration enabling rapid initiation of cellular repair through degradation of certain proteins. This is in contrast to S. stapfianus which requires several hours to replenish depleted ubiquitin transcripts. The ubiquitin response to drying and rehydration in evolutionarily diverse systems is characterized, and the role of repair mechanisms such as ubiquitin-mediated protein degradation in desiccation tolerance is assessed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006330623364
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of a desiccation-responsive small GTP-binding protein (Rab2) from the desiccation-tolerant grass Sporobolus stapfianus (1999)
    Springer
    Plant molecular biology 39 (1999), S. 809-821 
    Details
    ISSN: 1573-5028
    Keywords: desiccation tolerance ; differential display ; GTP-binding ; Rab2 ; Sporobolus stapfianus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used differential display to detect altering mRNA levels in response to desiccation and rehydration in leaves of the desiccation tolerant grass Sporobolus stapfianus. One of the RT-PCR products identified was used to isolate a cDNA of 999 bp which encodes a protein of 210 amino acids (predicted size 23 kDa). This protein displays considerable sequence similarity to mammalian and plant Rab2, a small GTP-binding protein, possessing several conserved motifs common to these regulatory proteins. Sporobolus Rab2 was expressed in Escherichia coli yielding a protein with an apparent molecular mass of ca. 30 kDa which was shown to have the ability to bind GTP. Rab2 transcript accumulated early in response to a decrease in relative water content (RWC) and remained high even in dried leaves. Rehydration of desiccated leaves resulted in a decrease in levels within 3 h of rewetting, with a brief increase at ca. 12 h. Accumulation of Rab2 transcript was also evident during drying and rehydration of the roots of S. stapfianus, as well as in leaves of the desiccation-sensitive grass Sporobolus pyramidalis. Earlier work on S. stapfianus concluded that the plant hormone ABA has little effect on inducing desiccation tolerance, however Rab2 transcript does exhibit a small increase in accumulation in response to exogenous ABA. A possible role for Rab2 with respect to desiccation tolerance and damage repair is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006183431854
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  • 3
    Electronic Resource
    Electronic Resource
    Regulation of juvenile hormone esterase gene transcription by juvenile hormone (1994)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 15 (1994), S. 391-400 
    Details
    ISSN: 0192-253X
    Keywords: Juvenile hormone ; juvenile hormone esterase ; transcription ; Trichoplusiani ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Juvenile hormone (JH) is a major hormone regulating insect development. We have obtained a cDNA and a genomic clone for juvenile hormone esterase (JHE), the enzyme that is involved in the degradation of juvenile hormone and which is critical for insect development. Analysis of the regulation of JHE during the final larval stadium in the cabbage looper, Trichoplusia ni, showed that the JHE mRNA levels are maximal on days 2 and 4 of the final stadium. Nuclear run-on analyses demonstrated that changes in JHE mRNA levels are primarily due to changes in the transcription rate of the gene, which may be a single copy in the genome. Treatment with a JH analog resulted in induction of JHE gene transcription, which could be detected within three hours after treatment. Salient features present in the 5′ flanking region of this JH-sensitive gene are presented, including the presence of sequences closely resembling binding sites for members of the family of nuclear receptors. This report is the first direct demonstration, by nuclear run-on analysis, of JH induction of gene transcription. © 1994 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020150502
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