ISSN:
1420-908X
Keywords:
Key words: IL-13 — MIP-1 α— Neutrophils — Inflammation — Chemokine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract. Objective and Design: To determine whether interleukin-13 (IL-13) possesses anti-inflammatory properties with respect to polymorphonuclear neutrophils (PMNs). Effects of IL-13 on production of the chemokine, macrophage inflammatory protein-1 alpha (MIP-1α), by PMNs were analyzed.¶Subjects: Human peripheral blood PMNs obtained from healthy volunteers.¶Methods: PMNs were stimulated by lipopolysaccharide (LPS) and/or IL-13 for selected periods of time, and MIP-1α expression was assessed by ELISA and Northern blot analysis.¶Results: IL-13 suppressed expression and production of PMN-derived MIP-1α mRNA and protein in a dose- and time-dependent manner. Inhibition of protein synthesis caused significant enhancement of MIP-1α mRNA expression. The inhibitory activity of IL-13 was abrogated, however, in the presence of cycloheximide, suggesting that IL-13's effect was via synthesis of de novo repressor protein(s).¶Conclusions: PMN-derived chemokines are regulated by both proinflammatory and immunomodulatory cytokines. The coordinated production of these substances is likely to be important in the orchestration of inflammatory and immune responses.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s000110050345
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