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  • 1
    ISSN: 1432-203X
    Keywords: Key wordsTriticum aestivum (L.) ; Anther culture ; Embryo induction ; Genotypic effects ; Growth temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ten soft white spring wheat (Triticum aestivum L.) F1 hybrids were grown under three temperature regimes, and anthers were cultured at two plating densities to investigate the effect of plant growth conditions, plating density, and genotype on embryo induction and plant regeneration. Anthers from plants grown at high temperature (25 °/18 °C) or from plants transferred from low (15 °/12 °C) to high temperature generally produced more embryos and green shoots, with a lower frequency of albinos, than did anthers from plants grown at low temperature. However, plating densities of 10 versus 20 anthers per milliliter, had little effect on anther response. Four of the five hybrids with `Fielder' as the female parent produced more embryos and green shoots than did hybrids with `AC Reed' as the female parent.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 2 (1983), S. 341-347 
    ISSN: 1573-5044
    Keywords: organogenesis ; in vitro selection ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A homogenization and plating technique is described which increases the number of shoots produced and decreases the time required for plant regeneration from callus cultures of birdsfoot trefoil. A 2- to 15-fold increase in the number of plants recovered per gram of callus is observed depending on the genotype. Characterization of a sample of the regenerated plants indicated no differences between plants from homogenized versus nonhomogenized callus for traits such as time of first flower, number of branches per plant, pollen stainability, stomate length, and whole plant yield. The technique has proven useful for efficient recovery of plants from long-term cultures and cultures selected for herbicide tolerance where a 15-fold increase in plant regeneration was obtained.
    Type of Medium: Electronic Resource
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