ISSN:
1662-8985
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
During the last decades, microbial bioleaching of metallic sulphide ores has become avery important process in the mining industry. The study of biodiversity in those systems hasrevealed the occurrence of several acidophilic organisms, forming a dynamic community includingBacteria and Archaea. Despite the importance of the heap bioleaching process, the metabolicbehaviour of a microbial community in bioleaching systems remains unknown. The role played bymembers of the community inhabiting a bioheap environment has been estimated by the phenotypiccharacteristics of pure cultures, such as iron oxidation, sulphur oxidation, resistance to high ionconcentration and metal tolerance, among others.The global genomic expression of the community inhabiting an industrial bioheap of low-gradecopper sulphide ore at Escondida mine in Chile was investigated by random arbitrary primedpolymerase chain reaction (RAP-PCR). Three random primers were used and twenty differentiallyexpressed bands were cloned and sequenced.Several sequences were related to 16S rRNA of members of the microbial community; twosequences were related to the α subunit of pyruvate dehydrogenase from Acidithiobacillusferrooxidans. The expression levels of the pyruvate dehydrogenase gene in samples from anindustrial bioleaching operation at different stages were checked by quantitative real-time PCR
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.435.pdf
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