ISSN:
1432-072X
Keywords:
Key wordsAcidianus brierleyi
;
Reductive tricarboxylic
;
acid cycle
;
3-Hydroxypropionate cycle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The autotrophic CO2 fixation pathway in Acidianus brierleyi, a facultatively anaerobic thermoacidophilic archaebacterium, was investigated by measuring enzymatic activities from autotrophic, mixotrophic, and heterotrophic cultures. Contrary to the published report that the reductive tricarboxylic acid cycle operates in A. brierleyi, the enzymatic activity of ATP:citrate lyase, the key enzyme of the cycle, was not detected. Instead, activities of acetyl-CoA carboxylase and propionyl-CoA carboxylase, key enzymes of the 3-hydroxypropionate cycle, were detected only when A. brierleyi was growing autotrophically. We conclude that a modified 3-hydroxypropionate pathway operates in A. brierleyi.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01682981
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