ISSN:
0173-0835
Keywords:
Two-dimensional polyacrylamide gel electrophoresis
;
Lead toxicity
;
Rabbit myocardium
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Despite reported adverse effects, the cardiovascular toxicity of lead remains controversial. The purpose of the present study was to determine if low-level subchronic exposure of rabbits to lead would produce detectable, concentration-dependent changes in myocardial proteins. Lead was administered to male Dutch Belted rabbits as a lead acetate solution, adjusted weekly to achieve and maintain the target blood lead levels of 0, 20, 40, and 80 μg/dL for 15 weeks. Lead exposures did not affect heart or body weights. Myocardial cocentrations of lead at sacrifice were 58 ± 25, 69 ± 23, 102 ± 62, and 105 ± 37 ng/g. Of 808 individual proteins resolved by two-dimensional electrophoresis (2-DE) in ventricular homogenates, 162 had coefficients of variation 〈 20%. A number of proteins were tentatively identified based on coordinate positions homologous to other established 2-DE patterns. Despite variable expression of some protein spots, none of the protein abundances analyzed were found to be significantly altered (P 〈 0.001) by the lead exposures studied. Therefore results show no detectable effect of a low-body burden of lead on major myocardial proteins of the rabbit.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150181540
Permalink