ISSN:
1573-5044
Schlagwort(e):
cotton
;
ovule epidermis
;
protoplasts
;
cell wall regeneration
;
β-1,3-glucans
;
β-1,4-glucans
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Culture protocols were developed and characterization of the regenerated cell walls was performed for protoplasts of cotton (Gossypium hirsutum L., L., var. Acala SJ-2) ovule epidermal cells. This work was undertaken in order to extend studies concerning nutritional effects and regulation of nucleotide sugar incorporation into β-1,3- and β-1,4-glucan components of cotton fiber cell walls. Protein and carbohydrate polymers and recovered from the culture medium. Analysis of a cellular fraction indicated that the majority of 14C incorporated from [14C] glucose was present in the hot-water-soluble fraction of the cells. The majority of label incorporated into cell wall material could be solubilized with acetic-nitric reagent, indicative of noncellulosic material, and characterized as β-1,3-linked glucans. Only 5 to 15% of the regenerated cell wall could be characterized as β-1,4-linked glucose indicative of cellulose.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00037758
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