ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract: The Na+,K+-ATPase α3 isoform has recently been demonstrated immunochemically in human brain. Conclusive biochemical evidence, however, is still lacking. In this study, a unique 50-kDa polypeptide, which is known to be specific to the rat α3 isoform, has been found in human brainstem Na+,K+-ATPase following formic acid treatment of the purified a isoform proteins. Human α3 Na+,K+-ATPase is also highly sensitive to ouabain inhibition, with a 50% ouabain inhibition value of 1.0 X 10−7 M. These results provide clear and direct evidence for the existence of the α3 isoform in human brain. Na+,K+-ATPase-α1, α2, and α3 isoforms—Human and rat brainstem axolemma—Ouabain sensitivity—Formic acid treatment. Peng J. H. F. et al. Highly ouabain-sensitive α3 isoform of Na+,K+-ATPase in human brain. J. Neurochem.58, 1180–1183(1992).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1992.tb09380.x
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