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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    International journal of food science & technology 11 (1976), S. 0 
    ISSN: 1365-2621
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ultrastructure of tapioca starch granules was studied using the freezeetching technique in an attempt to overcome the problems associated with the other methods of preparation for electron microscopy. A chemically-modified tapioca starch was also studied to ascertain whether chemical modification affected granule structure. Variations in fracture faces were observed in both modified and unmodified granules suggesting that organization within the granules was not homogenous. Particles were present on granule fracture faces. the size range of the particles was 4 to 10 nm in the unmodified and 6 to 15 nm in the modified granules. These observations were similar to those found by others in freeze-etched starch granules and must be considered to be of some structural significance. In contrast to the fracture face, the outer surface of the granules was smooth and particles were not evident.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 79 (1974), S. 59-91 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructure of immature phloem was studied in twelve species in seven genera of palms. A sieve element can be first recognized by the appearance of cuneate, electron dense inclusions in the plastid. Nacreous walls of an immature sieve element have microfibrils that are oriented perpendicular to the long axis of the cell. The most recently formed cellulose microfibrils are oriented parallel to the cortical microtubules. Cytoplasmic microfilaments 50–60 Å in diameter are often present in procambial as well as differentiating phloem cells. Such microfilaments occur in bundles and are usually oriented parallel to the long axis of the cell. In addition to the microfilaments, randomly arranged P-protein filaments 70–120 Å are also present in the differentiating sieve elements of some palms. The amount and the presence or absence of P-protein is very variable. The possible reasons for the sporadic occurrence and the variation in the quantities of P-protein in differentiating sieve elements of palms are discussed. Some structural differences between companion cells and parenchyma cells are evident.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Nicotiana (phloem unloading) ; Phloem unloading ; Photoassimilate unloading ; Translocation (phloem unloading)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A morphometric analysis of developing leaves of Nicotiana tabacum L. was conducted to determine whether imported photoassimilates could be unloaded by symplastic transport and whether interruption of symplastic transport could account for termination of import. Five classes of veins were recognized, based on numbers of cells in transverse section. Photoassimilate is unloaded primarily from Class III veins in tissue nearing the end of the sink phase of development. Smaller veins (Class IV and V) do not transport or unload photoassimilate in sink tissue because the sieve elements of these veins are immature until after the tissue stops importing. In Class III veins the sieve element-companion cell (SE-CC) complexes are surrounded by phloem parenchyma which abuts the bundle sheath. Along the most obvious unloading route, from SE-CC complex to phloem parenchyma to bundle sheath to mesophyll cells, the frequency of plasmodesmata at each interface increases. To determine whether this pattern of plasmodesmatal contact is consistent with symplastic unloading we first demonstrated, by derivation from Fick's law that the rate of diffusion from a compartment is proportional to a number N which is equal to the ratio of surface area to volume of the compartment multiplied by the frequency of pores (plasmodesmata) which connect it to the next compartment. N was calculated for each compartment within the vein which has the SE-CC complex as its center, and was shown to be statistically the same in all cases except one. These observations are consistent with a symplastic unloading route. As the leaf tissue matures and stops importing, plasmodesmatal frequency along the unloading route decreases and contact area between cells also decreases as intercellular spaces enlarge. As a result, the number of plasmodesmata between the SE-CC complex and the first layer of mesophyll cells declines in nonimporting tissue to 34% of the number found in importing tissue, indicating that loss of symplastic continuity between the phloem and surrounding cells plays a role in termination of photoassimilate unloading.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2048
    Keywords: Actin ; Allergen ; Cytoskeleton ; Lycopersicon ; Profilin ; Tissue printing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Profilin is a G-actin monomer-binding protein which has been shown to participate in actin-based tipgrowth of animal cells. The abundance of profilin in pollen and its occurrence in several vegetable foods raises the question of the role of profilin in plants. First, its distribution throughout various parts of the plant needs to be determined. This paper describes observations on the presence of profilin in the tomato plant (Lycopersicon esculentum Mill.). The distribution of profilin in flower buds, stems, leaves, roots, and fruits of tomato was determined by immunoblotting and by tissue printing, showing that profilin is present in most if not all parts of the tomato plant.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Wood science and technology 10 (1976), S. 247-271 
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The fine structure of differentiating and mature sieve elements, tracheary elements and fibers of palms has been studied. The fine structure of sieve elements in general resembles that of the sieve elements in angiosperms except that not all the species of palms have P-protein. Material examined after taking precautions to minimize sudden release of pressure in the phloem indicate that the sieve-plate pores may be free of occlusions in intact sieve elements. Sieve elements in the basal parts of the stem estimated to be several decades old maintain their structural integrity and apparently remain functional throughout the life of the palm. Differentiating xylem elements are characterized by the presence of amoeboid plastids with electron-dense granular stroma. All the organelles normally present in meristematic cells are also present in differentiating tracheary elements. Bundles of microfilaments are often present in such elements. Structural changes that occur as the tracheary element matures are comparable to those observed in tracheary elements of other angiosperms. Xylem parenchymatous cells do not have the so called “protective wall layer.” The most striking ultrastructural feature in differentiating fibers of the root is the deep invagination of plasmalemma that traverses almost the entire width of the cell. The secondary wall of the fibers is characteristically multilayered.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 22 (1992), S. 245-249 
    ISSN: 0886-1544
    Keywords: rhodamine phalloidin ; anti-chicken actin antibody ; plant actin ; DNase I ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Previous reports about phalloidin binding to plant actins have been indirect. We present here evidence showing that phalloidin does bind and stabilize filaments of actin extracted from pea roots. Criteria for the presence of actin included stabilization as a polymer in the presence of phalloidin, cross-reaction with antibody against chicken actin, affinity binding to DNase I, and ability to be decorated by the S1 fragment of rabbit muscle myosin.Phalloidin was able to stabilize polymers in pea root extracts against dissociation during SDS gel electrophoresis, and these polymers were shown to be composed exclusively of actin. Pea root actin isolated by affinity chromatography on a DNase I column was incubated with rhodamine phalloidin and electrophoresed on a native gel. The rhodamine fluorescence remained with the stabilized filaments, indicating clearly that phalloidin does bind to actin from a plant source. © 1992 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 29 (1994), S. 339-344 
    ISSN: 0886-1544
    Keywords: microfilament ; phalloidin ; immunoblotting ; immunocytochemistry ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Anti-actin monoclonal antibodies were prepared using phalloidin-stabilized actin that was purified from pea roots by DNase I affinity chromatography. One monoclonal antibody, designated mAb3H11, bound plant actin in preliminary screenings and was further analyzed. Immunoblot analysis showed that this antibody had a high affinity for plant actin in crude and purified preparations but a low affinity for rabbit muscle actin. In immunoblots of plant extracts separated on two-dimensional gels it appeared to bind all actin isoforms recognized by the JLA20 anti-chicken actin antibody. Using immunofluorescent cytochemistry, the antibody was used to observe actin filaments in aldehyde-fixed and methanol-treated tobacco protoplasts. These results indicate that mAb3H11 should be a useful reagent for the study of plant actins. © 1994 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 121 (1984), S. 54-64 
    ISSN: 1615-6102
    Keywords: Microfilament bundle distribution ; Vascular parenchyma ; Conifer roots ; Streaming cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The distribution of microfilament bundles (MFBs) in the primary tissues ofChamaecyparis obtusa roots has been investigated by electron microscopy. Nomarski differential interference-contrast (NDIC) images of MFBs in sections of embedded materials are also presented to complement the ultrastructural observations. The peripheral phloem parenchyma cells, also known as precursory phloem, generally possess greater numbers of MFBs than do any other cell type. MFBs are apparently absent in the cortical, meristematic or root cap tissues. The number of MFBs seen in a transection of a cell varies according to its position in the ontogenetic sequence. While all the MFBs in peripheral phloem parenchyma cells lie within 2.0 μm from and on occasion contact the plasmamembrane, some MFBs in other phloem and xylem cells are located in the central areas of the cytoplasm. The possible three-dimensional distribution of MFBs in a streaming peripheral phlowm parenchyma cell is discussed.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 165 (1991), S. 209-211 
    ISSN: 1615-6102
    Keywords: Actin ; Microfilaments ; Microtubules ; Preprophase band ; Tobacco ; High pressure freezing ; Freeze substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization and distribution of microfilaments (MFs) in the preprophase bands (PPBs) of tobacco (Nicotiana tabacum L. var. Maryland Mammoth) root tip cells were studied with high pressure freezing and freeze-substitution methods. MFs were present predominantly as single filaments interspersed among microtubules (MTs) throughout the PPB. Some MFs appeared to be associated with MTs, whereas others were not. This is the first time that MFs have been demonstrated in the PPB at the electron microscope level.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 165 (1991), S. 96-105 
    ISSN: 1615-6102
    Keywords: Cytoskeleton ; Microfilaments ; Actin ; Microtubules ; Nicotiana tabacum ; Root cap ; Leaf ; Cryofixation ; Propane jet freezing ; Freeze substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization and distribution of microfilaments in freze substituted leaf tissues and root tips of tobacco plants (Nicotiana tabacum L. var. Maryland Mammoth) were investigated in detail. Three categories of microfilaments were recognized in interphase cells of all tissues including those in the root cap: (1) microfilament bundles; (2) single microfilaments; (3) cortical microtubuleassociated microfilaments. While the microfilament bundles appeared to be distributed throughout the cytoplasm, the single microfilaments were mainly confined to the cell periphery. All three categories of microfilaments were associated with various organelles. Our study indicates that the three categories of microfilaments are normal cytoskeletal components in higher plant cells. The implications of these findings are discussed.
    Type of Medium: Electronic Resource
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