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  • 1
    Electronic Resource
    Electronic Resource
    Composition of cultivated mushrooms (Agaricus bisporus) during the growing cycle as affected by the nitrogen source introduced in composting (1968)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 16 (1968), S. 517-519 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf60157a037
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  • 2
    Electronic Resource
    Electronic Resource
    Changes in carbohydrate and enzyme levels during development of leaves of Prunus persica, a sorbitol synthesizing species (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 83 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Carbohydrate levels and activities of enzymes associated with starch, sucrose and sorbitol metabolism were assayed in leaves of peach [Prunus persica (L.) Batsch cv. Redhaven] of different ages, in order to examine developmental changes in leaf carbohydrate metabolism. Dry matter, soluble protein, chlorophyll and the activities of key enzymes of the reductive pentose phosphate pathway increased during leaf development. The levels of leaf carbohydrates, especially sorbitol and starch, also increased. Changes of starch levels were related to increases in the activities of enzymes associated to starch metabolism, such as ADPglucose-pyrophosphorylase (E.C. 2.7.7.27) and amylase (E.C. 3.2.1.1. plus E.C. 3.2.1.2). The activities of enzymes involved in sucrose and sorbitol degradation decreased during leaf development, whereas the activities of aldose-6-phosphate reductase (E.C. 1.1.1.200) and cytosolic fructase-1,6-bisphosphatase (E.C. 3.1.3.11) increased. In contrast, the activity of sucrose-phosphate synthase (E.C. 2.4.1.14) did not vary in a significant manner. The results suggest that the ability to synthesize and utilize both sucrose and sorbitol changes as peach leaves mature, and also that there are differences in metabolism of these two transport sugars during leaf development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb02478.x
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  • 3
    Electronic Resource
    Electronic Resource
    Evaluation of UV-Induced Mutants of Chlorella vulgaris for Single Cell Protein I. The Screening Effect of Cycloheximide and 6-Methylpurine (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 29 (1973), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Chlorella vulgaris was irradiated with UV doses allowing a 0.1 per cent survival. Dark recovery and photoreactivation were carried out either in the presence or in the absence of an inhibitor of protein synthesis. Faster growing and faster greening colonies were selected, and the amino acid composition of the mutant strains were evaluated. Higher growth and photosynthetic rates, higher chlorophyll content and lower respiration rate were shown by all mutants irrespective of the selection procedure. Selection against cycloheximide led to higher protein and RNA, but lower carotenoid content, whereas mutants selected against 6-methylpurine showed, in addition to a protein and carotenoid increase, a more favourable protein: RNA ratio.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1973.tb04800.x
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  • 4
    Electronic Resource
    Electronic Resource
    Developmental changes of enzymes of malate metabolism in relation to respiration, photosynthesis and nitrate assimilation in peach leaves (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 89 (1993), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The developmental profile of the activities of some enzymes involved in malate metabolism, namely phosphoenolpyruvate carboxylase (PEPC; EC 4. 1. 1. 31), NAD+-linked (EC 1. 1. 1. 37) and NADP+-linked (EC 1. 1. 1. 82) malate dehydrosenase (MDH), NAD+linked (EC 1. 1. 1. 39) and NADP+-linked (EC 1. 1. 1. 40) malic enzyme (ME), has been determined in leaves of peach [Prunus persica (L.) Batsch cv. Maycrest], a woody C3 species. In order to study the role of these enzymes, their activities were related to developmental changes of photosynthesis, respiration, and capacity for N assimilation. Activities of PEPC, NAD(P)+-MDH and NADP+-ME were high in young expanding leaves and decreased 2- to 3-fold in mature ones, suggesting that such enzymes play some role during the early stages of leaf expansion. In leaves of peach, such a role did not seem to be linked to C3 photosynthesis or nitrate assimilation, in that photosynthetic O2 evolution and activities of nitrate reductase (EC 1. 6. 6. 1) and glutamine synthetase (EC 6. 3. 1. 2) increased during leaf development. In contrast, leaf respiration strongly decreased with increasing leaf age. We suggest that in expanding leaves of this woody species the enzymes associated with malate metabolism have anaplerotic functions, and that PEPC may also contribute to the recapture of respiratory CO2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1993.tb01788.x
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  • 5
    Electronic Resource
    Electronic Resource
    Protein Electrophoretic Pattern of Two Chlorella Mutants with Low and High Content of Sulphur-Amino Acids (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 32 (1974), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two mutants of Chlorella vulgaris characterized by high (HS) or low (LS) content of sulphur-amino acids were compared to the wild strain with respect to their protein electrophoretic pattern.Three new bands, not present in the LS and wild strains, were found in the HS mutant. In algae grown in the presence of 35S labelled sulphate two of these new bands showed a very high 35S specific activity. Some electrophoretic bands common to LS, HS, and wild strain, were present in different proportions and showed different levels of specific radioactivity for each strain. Therefore, mutational events appear to have affected both the amino acid composition of single proteins and the relative amount of proteins with high and low sulphate content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1974.tb03143.x
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of decreased irradiance on N and C metabolism in leaves and roots of maize (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 91 (1994), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effects of decreased irradiance on fresh and dry weight, root respiration, levels of carbohydrates and N-compounds, and extractable activities of enzymes involved in C and N metabolism were evaluated in maize (Zea mays L. cv. Plauto) seedlings during the 7 days following transfer from 450 to 200 μmol m−2 s−1 PAR. The fresh weight of roots and stems, the initiation of new leaves, root respiration rate, and the accumulation of dry matter, soluble sugars, starch, malate and amino acids in both leaves and roots were strongly reduced at low irradiance. In contrast, the level of nitrate was increased in leaves and only marginally affected in roots. Leaf phosphoenolpyruvate carboxylase (EC 4.1.1.31) activity started to decrease after 24–34 h, whereas ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) activity and chlorophyll content were unaffected or only slightly reduced. In both leaves and roots, the adjustment of N metabolism to low irradiance occurred through a relatively rapid (30% after 10 h) and large (60% after 3 days) decrease of nitrate reductase (NR; EC 1.6.6.1) activity, followed by slower and smaller changes in the activity of nitrite reductase (EC 1.7.7.1), glutamine synthetase (EC 6.3.1.2) and NAD-dependent glutamate dehydrogenase (EC 1.4.1.2). We suggest that the preferential decrease of NR activity relative to other N-assimilating enzymes may be important for preventing the accumulation of toxic N-compounds like ammonia in both leaf and root tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb00661.x
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  • 7
    Electronic Resource
    Electronic Resource
    Sulphate Uptake in Two Mutants of Chlorella vulgaris with High and Low Sulphur Amino Acid Content (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 35 (1975), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two mutants of Chlorella vulgaris characterized by higher and lower content of sulphur amino acids compared with the wild strain were assayed for the efficiency of the sulphate uptake mechanism. In both mutants uptake exhibited positive cooperation kinetics and was strongly stimulated by sulphate starvation. Stimulation was depressed by cysteine and to a higher extent by methionine. Mutations affected the uptake efficiency concordantly with the level of sulphur amino acids. Addition to the starved strains of sulphate or chromate reduced the induced transport to the lower values of the non-starved strains. Addition of cycloheximide during the induction period prevented a further enhancement of transport without depressing the attained rate in the low sulphur mutant; it was followed by a rapid fall to the non-induced rate in the high sulphur mutant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1975.tb03913.x
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  • 8
    Electronic Resource
    Electronic Resource
    Light-modulation of nitrate reductase activity in leaves and roots of maize (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 94 (1995), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In order to examine the light-modulation of nitrate reductase (NR: EC 1. 6.6.1) activity in tissues of maize (Zea mays L. cv. Plauto). the enzyme was assayed under both selective (5 mM, Mg2+) and unselective conditions (2 mM EDTA; Vmax). in leaf and root extracts from seedlings transferred from high irradiance (HI: 450 μmol m−2s−1 PAR) to shade (200 μmol m−2 s−1) or to darkness. The corresponding changes in tissue nitrate were also evaluated. The results show that maize leaf NR activity (NRA) is subjected to reversible light-modulation involving rapid changes in the sensitivity of the enzyme to inhibition by Mg2+, In fact, NR assayed in the presence of Mg2+ lost about 60% and 20% of its activity after 30 min in the dark and in the shade, respectively. Vmax started to decrease only after 1–3 h. depending on the extent of light reduction. Deactivation in the shade was reversed in vivo by returning the plants to HI. the kinetics of reactivation being similar to those of deactivation. Following dark-deactivation in vivo, spontaneous reactivation of leaf NR occurred during in vitro incubation at 25°C. and the process was accelerated by AMP. The NR from HI-leaves was deactivated (half-time 2 min) by incubation with MgATP. but the extent of the in vitro deactivation was small. Maize root NR was more sensitive to Mg2+ than leaf NR, with 5 mM Mg2+ causing a 50% inhibition of the activity in HI-roots. In contrast to leaves, marked changes in the Mg2+-sensitivity of maize root NRA became apparent only after 3 h in the dark or in the shade, when Vmax had also declined significantly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1995.tb05316.x
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  • 9
    Electronic Resource
    Electronic Resource
    Chemical composition of papaya seeds (1981)
    Springer
    Plant foods for human nutrition 31 (1981), S. 77-83 
    Details
    ISSN: 1573-9104
    Keywords: endosperm ; chemical composition ; papaya seeds ; fatty acids ; sarcotesta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seeds of papaya cultivated in Somalia, which accounted for about 16% of the fresh fruit weight, were divided into sarcotesta and endosperm. Sarcotesta showed higher percentages of ash, crude protein, and crude fiber than did endosperm, but was lacking in fat. In contrast, endosperm contained 60% fat. Oil extract showed very high levels of oleic and palmitic acids. The essential amino acid profiles of endosperm and sarcotesta protein were determined and compared with the provisional FAO essential amino acid profile. The results indicated that the endosperm protein was a good potential source of supplemental protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01093891
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