ISSN:
1617-4623
Keywords:
Gene library
;
Sulfate-reducing bacteria
;
Proline biosynthesis
;
Plasmid stability
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A library of Deusulfovibrio desulfuricans Norway genomic DNA was constructed in Escherichia coli with pBR322 as vector and plasmids able to complement the proA and leuB mutations of the host were screened. It was observed that all the plasmids studied were highly unstable, the insert DNA being rapidely lost under non-selective growth conditions. A 2.75 kb DNA fragment of D. desulfuricans Norway was found to complement E. coli ProA, ProB and ProC deficiencies. From the results of restriction analysis and Southern hybridization, it is proposed that the genes involved in proline and leucine biosynthesis are clustered on the chromosome of D. desulfuricans Norway.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00326549
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