ISSN:
1432-1017
Keywords:
laser light scattering
;
microscopy
;
acinar cells
;
granule secretion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract We report here the direct observation of the motion of cytoplasmic subcellular organelles and macromolecules within single, metabolically active, pancreatic acinar cells using microscope laser-light scattering spectroscopy (MLLSS). The relative amount and the “effective diffusion coefficient” of the mobile particles shows a marked difference in magnitude between the apical and basal regions of the cell. Secretory stimulation evoked by the cholinergic agonist bethanechol induces changes in the relative motion in the cytoplasm of approximately one fifth of the acinar cells. This study demonstrates the feasibility and utility of a novel technique, MLLSS, for the analysis of intracellular events in regions as small as (2 μm)3 in single, granule secreting cells. It also shows MLLSS to be a powerful tool for the detection and measurement of altered motion in disrete subcellular regions of small mammalian cells after biochemical and pharmacological manipulations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00257499
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