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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural study of the endocrine cells of the gut of Testudo graeca (Chelonia) (1989)
    Springer
    Anatomy and embryology 180 (1989), S. 103-108 
    Details
    ISSN: 1432-0568
    Keywords: Ultrastructure ; Gut ; Endocrine cells ; Testudo graeca ; Chelonia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The digestive tract of Testudo graeca (Chelonia) was investigated by means of electron microscopy using both conventional and immunocytochemical techniques. EC-, L-, D-, G-, B-, N- and EC-L-cells were detected. These cells share several common ultrastructural characteristics with the endocrine cells of mammals (i.e. clear cytoplasm, prominent Golgi apparatus, secretory granules etc.). EC and D1 cells have so far not been described in the esophagus of any animal species; in the present study these cells have been observed in the esophagus of T. graeca. Of special interest was the presence of B-cells in the intestine, suggesting that the migration of B-cells from the gut to the pancreas to constitute pancreatic islets is not concluded in T. graeca. The present study demonstrates that the gut endocrine system of T. graeca is a complex structure containing a large variety of endocrine cell types similar in morphology to those found in higher vertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00321906
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative immunohistochemical study of the gastroenteropancreatic endocrine system of three reptiles
    Amsterdam : Elsevier
    General and Comparative Endocrinology 76 (1989), S. 171-191 
    Details
    ISSN: 0016-6480
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0016-6480(89)90148-2
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Histochemistry of glycoconjugates in the gallbladder epithelium of ten animal species (1989)
    Springer
    Histochemistry and cell biology 91 (1989), S. 437-443 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A battery of seven lectins and several conventional mucin histochemical techniques were used to identify the epithelial mucins of the gallbladder of ten species: man, rabbit (Oryctolagus cuniculus, mammalia), hamster (Mesocricetus auratus, mammalia), chicken (Gallus gallus, bird), sparrow (Passer domesticus, bird), moorish gecko (Tarentola mauritanica, reptilia), ladder snake (Elaphe scalaris, reptilia), lake frog (Rana perezi, amphibia), natterjack toad (Bufo calamita, amphibia) and gilthead sea bream (Sparus auratus, fish). Glycogen was found in the epithelial lining of the reptilian and amphibian gallbladders. Sulphate and carboxyl groups were frequently found in the same species, except in the ladder snake and natterjack toad gallbladders where only sulphate groups were identified. Sialic acid residues were detected in man, rabbit, bird, T. mauritanica, R. perezi and fish gallbladders. ConA binding pattern was similar in the ten species studied. In the human gallbladder only PNA failed to label the luminal surface, while the glands were only unreactive to DBA. The human gallbladder showed a large variety of saccharides. The present results suggest that no relation exists between the composition of the gallbladder mucins and the situation of the species in the phylogenetic scale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493832
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Immunolocalization of Transforming Growth Factor-α and Epidermal Growth Factor Receptor in the Rat Gastroduodenal Area (1999)
    Springer
    Digestive diseases and sciences 44 (1999), S. 1408-1416 
    Details
    ISSN: 1573-2568
    Keywords: TGF-α ; STOMACH ; DUODENUM ; IMMUNOHISTOCHEMISTRY
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The maintenance of gastrointestinal epitheliumintegrity requires a fine balance between proliferationand differentiation as well as protection againstgastric acid secretion. Transforming growthfactor-α (TGF-α) regulates these functions bybinding to epidermal growth factor receptor (EGF-R).This study was designed to identify the localization ofTGF-α and EGF-R in the rat gastroduodenal region. In the stomach, the surface and gastric pitcells showed staining for TGF-α antibodies in thecytoplasm and basolateral and apical membranes.TGF-α and EGF-R were observed in the supranuclearregion of the cells lining the gland. In the duodenum,the enterocytes coexpressed both TGF-α and EGF-Rin the supranuclear area. The EGF-R was also observed inthe apical membrane. Brunner's glands were positive for both TGF-α and EGF-R antibodies. Ourresults demonstrate the coexpression of TGF-α andEGF-R in the rat gastroduodenal area, which suggests afunctional role for them in the establishment and maintenance of the epithelialrenewal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026655903294
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  • 5
    Electronic Resource
    Electronic Resource
    Immunohistochemical localization of transforming growth factor-α and epidermal growth factor-receptor in the mesonephros and metanephros of the chicken (1993)
    Springer
    Cell & tissue research 271 (1993), S. 3-8 
    Details
    ISSN: 1432-0878
    Keywords: Transforming growth factor-alpha ; Epidermal growth factor-receptor ; Mesonephros ; Metanephros ; Domestic fowl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Transforming growth factor-alpha (TGF-α) is a polypeptide related to epidermal growth factor (EGF). Both bind to EGF-receptor (EGF-R) to carry out their function in a variety of tissues and cell lines. Several studies have shown their presence in mammalian kidney, however, nothing has to date been stated concerning their existence in avian kidney. Expression of TGF-α and EGF-R is reported here for the first time during the development of the chicken kidney. Using immunohistochemical techniques, we identified a TGF-α (but not EGF) in mesonephric distal tubule cells from day 8 to day 20 of embryonic development and in metanephric distal tubule cells from day 14 of embryonic development to the adult. The histochemical characteristics of these cells and their histological localization suggest that they may be the “principal cells” of the distal tubules. Similarly, EGF-R was found in mesonephric proximal tubule cells from day 7 to day 18 of embryonic development and in metanephric proximal tubule cells from day 13 of embryonic development up to adult stages. The coexistence of both TGF-α and EGF-R from the onset of development of mesonephros and metanephros supports their possible role in mechanisms of proliferation and differentiation of the cells of these organs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00297536
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of serotonin in the reptilian lung (1987)
    Springer
    Cell & tissue research 248 (1987), S. 713-715 
    Details
    ISSN: 1432-0878
    Keywords: Pulmonary neuroendocrine cells ; Serotonin ; Neuroepithelial bodies ; Argyrophil cells ; Reptiles (Lacerta lepida, Mauremys caspica, Pseudemys scripta elegans, Testudo graeca)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of serotonin-immunoreactive cells in the lung of 4 species of reptiles was investigated. Serotonin-containing cells were found forming groups in the interconnecting septa in all 4 species studied, and also as solitary cells in Testudo graeca and Mauremys caspica. Serotonin-containing cells were also localized in the intramural ganglia of Pseudemys scripta elegans and Testudo graeca. The present study confirms that serotonin is widely distributed in the lung of vertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216504
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  • 7
    Electronic Resource
    Electronic Resource
    Histochemical and ultrastructural study of the digestive tract of the tortoise Testudo graeca (Testudines) (1990)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 204 (1990), S. 235-245 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The digestive tract of the tortoise Testudo graeca (Testudines) was investigated by means of light and electron microscopy. The esophagus of T. graeca was lined by two types of epithelium: non-keratinized stratified squamous in the upper portion and stratified columnar in the lower. The lamina propria of the esophagus contained tubular or tubuloacinar glands. The mucosa of the stomach showed similar characteristics to those of other reptiles. The small intestine exhibited longitudinal folds lined by absorptive and goblet cells. The large intestine was lined by columnar mucous cells. Within the lamina propria of the large intestine, masses of 10-15 epithelial cells connecting with the surface epithelium by means of slender cytoplasmic processes were observed. A battery of six lectins (Con-A, PNA, WGA, DBA, SBA, and LTA) was used to identify the epithelial mucins. WGA and DBA reacted with all types of mucous cells throughout the alimentary canal. PNA was only unreactive in the intestine, LTA in the esophagus, and SBA in the large intestine. These results indicate a similar lectin-binding pattern throughout the gut of T. graeca.
    Additional Material: 19 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052040302
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