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  • 1
    ISSN: 1431-4630
    Keywords: Key words Two-dimensional electrophoresis ; Isoelectric focusing ; Flat fish ; Protein ; Identification ; Differential characterization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The water-soluble proteins of nine flat fish species of high commercial value, belonging to the Pleuronectidae, Scophtalmidae, and Soleidae families, were analyzed by two-dimensional (2D) electrophoresis, this being carried out by nondenaturing isoelectric focusing (IEF) in the 3.5–9.5 pH range and gradient SDS-PAGE in the 12–14% range. Most of the major proteins fell in the 3.5–6.9 pH range. From these, the most specific proteins were in the acidic fraction (pI〈5.2; MW〈16 kDa). Species-specific 2D protein patterns were observed in all the nine species, and more than 25 proteins could be individualized. The combination of nondenaturing IEF and gradient SDS-PAGE, both in the absence of urea, allowed detailed characterization of both the isoelectric points and molecular weights of the major water-soluble proteins and proved to be a valuable tool for the differential characterization of the flat fish species studied.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 204 (1997), S. 411-416 
    ISSN: 1431-4630
    Keywords: Key words Fish species ; Gadoids ; Identification ; HPLC ; Sarcoplasmic proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A simple and rapid method to identify raw or frozen gadoid fish species is described. The methodology is based on the HPLC separation of water-soluble proteins and monitoring at 230 nm. Chromatographic profiles were reproducible between individuals belonging to the same species; lower variations in the relative retention time than in peak area percentage were observed. Results obtained from an ANOVA test allowed the distinction of characteristic peaks. Profiles were independent of fish quality: fish refrigerated for 10 days showed the same HPLC protein profile as those that were fresh. The ability of the identification procedure was checked by analysis of a set of frozen commercial samples.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Manuscripta mathematica 38 (1982), S. 333-341 
    ISSN: 1432-1785
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract In his book (3), Pietsch presents the problem of a direct construction of the injective hull of an operator ideal on loca. lly convex spaces. We construct the infective hull of an arbitrary operator ideal on locally convex spaces using the functor , where and B cover the family of all equicontinuous subsets of E'(L is the category of all locally convex spaces). If the operator ideal is bounded, we ob tain its infective hull using seminorm ideals.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Trends in Food Science and Technology 4 (1993), S. 395-401 
    ISSN: 0924-2244
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sensory and quantitative microbiological analyses were performed in farmed turbot (Psetta maxima) during iced storage. Sensory analyses revealed a shelf life of 19 d for farmed turbot. The production of total volatile base nitrogen (TVB-N) and trimethylamine-nitrogen (TMA-N) was low and reached levels close to 40 mg TVB-N/ 100 g muscle and 3.5 mg /TMA-N/100 g muscle, even after 40 d of refrigerated storage. The pH value of turbot muscle increased from an initial value of 6.3 to close to 7.0 after 29 d of iced storage. Microbial growth was slow in iced turbot: total aerobic counts reached levels below 7 log colony forming units/g units even after 40 d of storage. Lactic acid bacteria—mainly Lactobacillus delbrueckii subsp. delbrueckii and Lactococcus lactis subsp. lactis strains—were predominant among the proteolytic strains isolated from iced turbot. Proteolytic strains of L. lactis subsp. lactis, Brochothrix thermosphacta, Plesiomonas shigelloides, Proteus vulgaris, and Pantoea species were also isolated from temperature-abused turbot, such proteolytic strains being predominant with respect to nonpro-teolytic microorganisms, suggesting a preferential role of such proteolytic bacteria in the spoilage of turbot. The slow bacterial growth, together with the relative predominance of lactic acid bacteria over Gram-negative microorganisms, may be related to the extraordinary maintenance of the quality and extended shelf life of farmed turbot.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : We investigated the effect of a commercial plant extract, Rosmol-P (RP) on the stability of fish during frozen storage. Horse mackerel (Trachurus trachurus) fillets were soaked in water (water control) and 2 RP concentrations (RP-1 = 0.333%; RP-2 = 1.333%) and compared with untreated fillets (blank control). Fluorescence detection and thiobarbituric acid index showed a lower oxidation development for both RP treatments than for both controls, especially in the case of the highest concentration (RP-2). The decrease in glutathione peroxi-dase activity was found to be slower in the case of the RP-2 treatment. The sensory analysis showed an increased shelf-life according to the sequence blank control 〈 water control 〉 RP-1, RP-2.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archiv der Mathematik 75 (2000), S. 370-375 
    ISSN: 1420-8938
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract. Let X and Y Banach spaces. Two new properties of operator Banach spaces are introduced. We call these properties “boundedly closed” and “d-boundedly closed”. Among other results, we prove the following one. Let ${\cal U}(X, Y)$ an operator Banach space containing a complemented copy of c 0. Then we have: 1) If ${\cal U}(X, Y)$ is boundedly closed then Y contains a copy of c 0. 2) If ${\cal U}(X, Y)$ is d-boundedly closed, then X * or Y contains a copy of c 0.
    Type of Medium: Electronic Resource
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