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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 28 (1989), S. 9116-9123 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The mutation causing Huntington disease (HD) has been identified as an expansion of a polymorphic (CAG) n repeat in the 5′ part of the huntingtin gene. The specific neuropathology of HD, viz. selective neuronal loss in the caudate nucleus and putamen, cannot be explained by the widespread expression of the gene. Since somatic expansion is observed in affected tissue in myotonic dystrophy, we have studied the length of the (CAG) n repeat in various regions of the brain. Although we have not found clear differences when comparing severely and mildly affected regions, we have observed a minor increase in repeat length upon comparison of affected brain samples with cerebellum or peripheral blood. Hence, although further somatic amplification seems to occur in affected areas of the brain, the differences between affected and unaffected regions are too small to make this mechanism an obvious candidate for the cause of differential neuronal degeneration in HD.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Nuclear Instruments and Methods in Physics Research Section A: 337 (1993), S. 34-43 
    ISSN: 0168-9002
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 9 (1998), S. 369-379 
    ISSN: 1572-9729
    Keywords: calcium ; fluoranthene fluoranthene ; membrane permeabilization ; polycyclic aromatic hydrocarbons ; Sphingomonas paucimobilis ; surfactant-enhanced biodegradation ; Triton X-100
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Surfactants are known to increase the apparent aqueous solubility of polycyclic aromatic hydrocarbons (PAHs) and may thus be used to enhance the bioavailability and thereby to stimulate the biodegradation of these hydrophobic compounds. However, surfactants may in some cases reduce or inhibit biodegradation because of toxicity to the bacteria. In this study, toxicity of surfactants on Sphingomonas paucimobilis strain EPA505 and the effect on fluoranthene mineralization were investigated using Triton X-100 as model surfactant. The data showed that amendment with 0.48 mM (0.3 g l-1) of Triton X-100 completely inhibited fluoranthene and glucose mineralization and reduced cell culturability by 100% in 24 h. Electron micrographs indicate that Triton X-100 adversely affects the functioning of the cytoplasmic membrane. However, in the presence of 4.13 mM Ca2+-ions, Triton X-100 more than doubled the maximum fluoranthene mineralization rate and cell culturability was reduced by only 10%. In liquid cultures divalent ions, Ca2+ in particular and Mg2+ to a lesser extent, were thus shown to be essential for the surfactant-enhanced biodegradation of fluoranthene. Most likely the Ca2+-ions stabilized the cell membrane, making the cell less sensitive to Triton X-100. This is the first report on a specific factor which is important for successful surfactant-enhanced biodegradation of PAHs.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1572-9729
    Keywords: biodegradation ; bioemulsifier ; biosurfactant ; polyaromatic hydrocarbons ; soil bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fifty-seven bacterial strains were isolated from PAH-contaminated soils using PAH-amended minimal medium. The isolates were screened for their production of biosurfactants and bioemulsifiers when grown in liquid media containing selected PAHs. The results suggest that many, but not all, of the isolates are able to produce biosurfactants or bioemulsifiers under the experimental conditions. The majority of the strains isolated on phenanthrene, pyrene, and fluoranthene were better emulsifiers than surface tension reducers and the stability of the formed emulsions was in general high. The strains isolated on anthracene were in general better in lowering the surface tension than in forming emulsions. In all strains, reduction of surface tension and emulsion formation did not correlate. However, in the majority of strains the two factors were associated with the bacterial cell surfaces, rather than the culture supernatants. Nevertheless, supernatants from selected surfactant-producing anthracene isolates increased the aqueous solubility of anthracene. Although a significant potential for surfactant and emulsifier production in the microbiota of the PAH-contaminated soils was found in this study, the ability of individual strains to mineralize PAHs did not coincide with production of surface-active compounds.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 166 (1994), S. 173-179 
    ISSN: 1573-5036
    Keywords: peptide hydrolases ; protein remobilisation ; senescence ; Triticum aestivum L. ; waterlogging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of waterlogging on leaf senescence and protein remobilisation was investigated in winter wheat (Triticum aestivum L., cv. Arina), which was grown in large pots embedded in the field in spring. The soil of intact pots was flooded permanently from anthesis to maturity, while in perforated control pots the soil was well aerated throughout the maturation period. No major effects of waterlogging were observed in the third leaf from the top, which was already senescing when the treatment was started. The degradation of proteins and chlorophyll was accelerated slightly in the second leaf from the top and considerably in the flag leaf. The contents of free amino acids in the uppermost leaf blades were lower on waterlogged soil than on control pots. Furthermore, amino acids in the blades did not accumulate during the earlier protein remobilisation observed on flooded soil, indicating that the export of amino acids via the phloem was fully functional. The loss of leaf proteins and the changes in the pattern of peptide hydrolases during senescence were similar in treated and control plants. Endopeptidase activities increased in an early phase of senescence at pH 5 and later also at pH 9, while aminopeptidase activities decreased. Therefore, nitrogen remobilisation was accelerated on flooded soil, but the senescence-related changes were very similar to those observed in control plants.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 160 (1994), S. 87-95 
    ISSN: 1573-5036
    Keywords: grain filling ; nutrient redistribution ; redox potential ; Triticum aestivum L. ; waterlogging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat plants (Triticum aestivum L., cv. Arina) growing in large pots (perforated at the bottom for controls, intact for flooding) were embedded in the field in spring. Waterlogging was initiated at anthesis and was maintained throughout the maturation period. Grain yield as well as potassium, phosphorus and magnesium contents in the shoot were decreased on flooded soil, while manganese and iron contents increased considerably. Total calcium and zinc contents per shoot remained comparable to those in controls. The reduction of potassium, phosphorus and magnesium contents by waterlogging was greatest in the grains, while manganese and iron accumulated mostly in the vegetative parts and the glumes. Zinc contents were also lowered in the grains during waterlogging due to an inhibited redistribution from the vegetative parts to the grains. Our results indicate that flooding caused not only an accumulation of manganese and iron in the shoot, but also affected the redistribution of macro- and micronutrients to the maturing gains.
    Type of Medium: Electronic Resource
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