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  • 1
    Electronic Resource
    Electronic Resource
    The debate on the presence of HIV-1 virus in human spermatozoa (1996)
    Springer
    Perspectives in drug discovery and design 5 (1996), S. 129-142 
    Details
    ISSN: 1573-9023
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The debate about the presence of HIV-1 virus particles in human spermatozoa is reviewed and recent experimental results are reported in detail. Using immunocytochemistry, in situ hybridization at the electron microscopy level, the polymerase chain reaction and in vitro fertilization, it has been demonstrated that human spermatozoa can incorporate HIV-1 using special receptors different from the commonly used CD4, and that the spermatozoa remain active and able to vehicle viral particles into the oocyte, which is regularly being fertilized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174008
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Submicroscopic mathematical evaluation of spermatozoa in assisted reproduction. 2. In vitro fertilization. (notulae seminologicae. 7) (1996)
    Springer
    Journal of assisted reproduction and genetics 13 (1996), S. 635-646 
    Details
    ISSN: 1573-7330
    Keywords: human spermatozoa ; electron microscopy ; mathematics ; in vitro fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This paper belongs to a series of applications of the Baccetti et al. formula (1) to the submicroscopical mathematical examination of human spermatozoa used for assisted reproduction. The present experiment concerns IVF, a technique requiring careful evaluation of sperm quality to predict the success of the program. Our results demonstrate that the sperm submicroscopic characters introduced in the formula are clearly correlated with the result of IVF. In fact the two numbers concerning sperm quality (percentage of spermatozoa free from structural defects and total number in the ejaculate of spermatozoa free from defects) obtained in successful and unsuccessful IVF groups, showed a large difference. Thet distribution in both cases reached a significance of 0.005. The synthetic parameters obtained are therefore a good tool in the prediction of sperm power in in vitro insemination techniques. The most important characteristics seem to be the quality of the acrosomal complex, the status of the chromatin, the shape of mitochondria, the axonemal pattern, and the membrane integrity. All these characteristics are expressed with largely different means in successful and unsuccessful ejaculates (t distribution significant at 0.005). All these data confirm that submicroscopic mathematical diagnosis offers a convincing evaluation of sperm structure and function, involving all organelles, including acrosome function and cell motility. It is also demonstrated that sperm quality is a major factor in the success of IVF and that it is clearly revealed by the integrity of the majority of the sperm organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02069642
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Crater defect in human spermatozoa (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 22 (1989), S. 249-255 
    Details
    ISSN: 0148-7280
    Keywords: human spermatozoa ; “crater defect,” ; nuclear malformation ; acrosome malformation ; cytoskeleton ; sperm head morphogenesis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: This report describes the “crater defect” in human spermatozoa, a malformation that consists of a nuclear and acrosomal invagination present in 100% of the cells, whereas tail structure and motility are fairly normal. The defect occurs during spermiogenesis. A possible concomitance with abnormalities in the microtubular apparatus involved in the sperm molding is discussed.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120220302
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  • 4
    Electronic Resource
    Electronic Resource
    Morphogenesis of the decapitated and decaudated sperm defect in two brothers (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 23 (1989), S. 181-188 
    Details
    ISSN: 0148-7280
    Keywords: human spermatozoa ; male infertility ; decapitated ; decaudated ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The “decapitated sperm” defect, found in both of two sterile brothers, may be assumed to have a genetic origin. The present material suggests that the term “decapitated spermatozoa” is not exact, because detached heads and tails were found in the brothers' ejaculate that could be regarded as “decapitated tails” and “decaudated heads.” The present report describes frequent, more or less advanced stages of detachment. Both heads and tails showed a normal structure in which only the postnuclear region was deficient, lacking basal plate and implantation fossa. A break at a different level of the midpiece, and therefore three kinds of separation, were observed. The defect, according to the present research, must originate in the testicular region, whereas the detachment occurs in the epididymis.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120230205
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    Paper (German National Licenses)
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