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Transforming growth factor-β2 (TGF-β2) reverses the inhibitory effects of fibrin sealant on cutaneous wound repair in the pig (2002)

Petratos, Peter B. ; Felsen, Diane ; Trierweiler, Grant ; [et al.]

Malden, USA : Blackwell Science Inc

Wound repair and regeneration 10 (2002), S. 0

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ISSN: 1524-475X

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Tensile strength of 2-cm, full-thickness, surgically incised porcine skin wounds sealed with fibrin sealant was enhanced compared to conventionally sutured wounds at 6 hours postwounding, but was significantly reduced after 3 days. Supplementation of fibrin sealant with transforming growth factor-β2 (TGF-β2) reversed the inhibitory effects of fibrin sealant on tensile strength at 3 days, and enhanced tensile strength at 7 days compared to suture or fibrin sealant alone. By 14 days, the tensile strengths of all wounds were similar, although wounds treated with fibrin sealant supplemented with TGF-β2 showed a small, but statistically significant, improvement in wound strength compared to wounds treated with fibrin sealant alone. Histological assessment at day 7 revealed significant remnants of fibrin sealant at the wound site following fibrin sealant treatment alone, while wounds treated with fibrin sealant supplemented with TGF-β2 or suture exhibited fibroblast infiltration and extracellular matrix deposition. At day 7, TGF-β was immunolocalized in the base and margins of only wounds treated with fibrin sealant supplemented with TGF-β2. A significant increase in matrix metalloproteinase-9 activity was found in fibrin sealant–treated wounds at day 7 as compared to sutured wounds. Addition of TGF-β to the fibrin sealant suppressed the up-regulation of matrix metalloproteinase-9 in these wounds. These results suggest that fibrin sealant supplemented with TGF-β may provide superior wound healing as compared to fibrin sealant alone. (WOUND REP REG 2002;10:252–258)

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1524-475X.2002.10409.x

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Effect of steroid hormones on endotoxin-mediated cartilage degradation (1988)

Hubbard, John R. ; Mattmueller, Danette R. ; Steinberg, James J. ; [et al.]

Springer

Molecular and cellular biochemistry 79 (1988), S. 31-37

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ISSN: 1573-4919

Keywords: cartilage ; degradation ; lipopolysaccharides ; steroid hormones

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: Summary Cartilage degradation is a characteristic feature of various types of human arthritis, notably rheumatoid arthritis and osteoarthritis. The influence of glucocorticoid and other steroid hormones on cartilage proteoglycan breakdown was examined in a model system in which breakdown is readily quantified by the release of proteoglycan from cultured bovine nasal cartilage discs. Endotoxin (bacterial lipopolysaccharides) treatment enhanced the depletion of cartilage proteoglycan by 2–3 fold. This was inhibited in a concentration-dependent manner by hydrocortisone (10−9 to 10−5M) or other glucocorticoid hormones (dexamethasone, prednisolone, cortisone). Inhibition required the continued presence of the steroid. Removal of hydrocortisone (3 × 10−7M) after 4 days from endotoxin-treated cultures resulted in the rapid restoration of an endotoxin response, so that proteoglycan release approached maximum levels during a second 4-day culture period. Other C-21 steroid hormones (progesterone, aldosterone) were also inhibitory at 10−5M, but testosterone and β-estradiol showed little influence on endotoxin action. Proteoglycan products of smaller average mol wt (Sepharose CL-2B chromatography), consistent with core protein cleavages, were released from endotoxin-treated cartilage. Cleavage was unaffected by β-estradiol, partially blocked by aldosterone and largely prevented by hydrocortisone administration.