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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 39 (1961), S. 53-57 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of different concentrations of CO2 on the germination of conidiospores of Aspergillus niger A 5 has been studied using Pardee's buffer mixtures which maintain constant CO2 tensions. The beneficial effect of CO2 on germination is maximum at 0.5% CO2 concentration, when 70–90% of the spores germinate within 6 hours, whereas in controls with air containing 0.03% CO2 there is only 15–20% germination at 6 hours. At higher CO2 concentrations this beneficial effect of CO2 on germination diminishes and at 3% there is a complete inhibition of spore germination. The spore density and the ph of the medium have a noticeable effect on germination rates in presence of 0.5% CO2. The germination rates decrease at spore densities higher than 5 · 105/ml and at a ph of 6.8.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 47 (1964), S. 338-343 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The oxidation of branched C5-dicarboxylates has been studied with microorganisms obtained from enrichement cultures with each of these acids. Bacteria grown on itaconate, mesaconate or methylsuccinate oxidize most of these compounds rapidly; (+) citramalate is oxidized 2–4 times as rapidly as (-) citramalate; and citraconate only feebly. Citraconate-grown cells do not oxidize itaconate and oxidize feebly mesaconate and methylsuccinate; they oxidize (-) citramalate 3–4 times faster than (+) citramalate. Cell-free extracts of various strains grown on different C5-dicarboxylates contain pronounced mesaconase activity, varying amounts of citraconase and hardly any itaconase activity.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 11 (1957), S. 317-338 
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 12 (1976), S. 15-22 
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Serratia marcescens Sa-3 possesses two homoserine dehydrogenases and neither has any aspartokinase activity unlike the case ofEs-cherichia coli enzymes. The two enzymes have been separated. One of them is active with either NAD− or NADP+ and has been purified about 180-fold to homogeneity. This enzyme is completely repressed by the presence of 1mm methionine or homoserine in the growth medium, but its activity is unaffected by any amino acid of the aspartate family either singly or together. In many of its properties (such as pH optimum, Km for substrate and cofactors), it resembles its counterpart inE. coli K12. Potassium ions stabilize the enzyme but are not essential for activity. Its molecular weight is around 155,000 as determined by gel filtration and approximately 76,000 by SDS-polyacrylamide gel electrophoresis. This suggests that the enzyme has two subunits (polypeptide chains) in the molecule: 8m urea has no effect on enzyme activity. This enzyme represents approximately 30% of the total homoserine dehydrogenase activity ofS. marcescens unlike inSalmonella typhimurium andE. coli K12 where it is a minor or a negligible component.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 1 (1975), S. 173-181 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Aspergillus carbonarius produces exocellular pectinolytic enzymes which are active within the acid range of pH and therefore are useful in commercial processing of fruits. The fungus produces pectin methylesterase, a viscosity-reducing enzyme, and exo-polygalacturonase; but it does not produce transeliminases. The optimum pH range and temperature for the above-mentioned enzyme activities are 3.5 to 4.0 and 50°, respectively. Enzymic hydrolysates of both pectin and pectic acid contained only monogalacturonate. The enzymes are stable at pH 3.0 to 4.5 at room temperature (20–30°) for more than a month. A preliminary purification yielded two fractions, both of which showed viscosity-reducing as well as saccharogenic activities. Pectin methylesterase was unaffected when treated with 6M urea for 5 hr at pH 6.7 and 25°, whereas polygalacturonase and viscosityreducing activities were completely inactivated.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant foods for human nutrition 28 (1979), S. 293-303 
    ISSN: 1573-9104
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Carbohydrate make-up of different varieties (ie, Hamsa, Purna and Indof) of Finger millet(Eleusine coracana) and Foxtail millet(Setaria italica) has been studied. All the millets contain about 63 to 70% total carbohydrates of which, based on the whole grains, free sugars account for 0.46 to 0.69%, starch 56.0 to 61.0% cellulose 0.70 to 1.80%, pentosans 5.50 to 7.20%. 70% ethanol-extractable sugars were xylose (1.5 to 4.3%), fructose (8.6 to 15%), glucose (9.9 to 15%), sucrose (31 to 35%), maltose (9 to 11.0%), raffinose (8.6 to 12%), maltotriose (5 to 6.1%), unidentified components (3 to 10%) and higher oligosaccharides (5to 9%). The watersoluble gums of the millets contain arabinose and xylose as the major sugar components together with minor amounts of mannose and galactose, and varying amounts of glucose. Hemicellulose A was found to consist mainly of glucans containing arabinose and xylose in minor amounts. Hemicellulose B contained arabinose and xylose with smaller amounts of glucose and galactose.
    Type of Medium: Electronic Resource
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