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  • 1
    Electronic Resource
    Electronic Resource
    Positive Feedback of Vasopressin on its Own Release in the Central Nervous System: in vitro Studies (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neuroendocrinology 2 (1990), S. 0 
    Details
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In this paper evidence is shown that synthetic arginine vasopressin (AVP) can evoke marked in vitro release of endogenous immunoreactive AVP (I-AVP) from male rat septal and hypothalamic tissue superfused in vitro. The stimulatory action was dosedependent with a maximal amplification factor of 2.3 when using 14 pg of synthetic AVP as the stimulus. It was highly specific since only AVP was effective and not three closely related substances such as lysine vasopressin, oxytocin and a 4–9 C fragment of AVP. This reproducible effect of AVP required, however, effective concentrations of bacitracin (10−4 to 10−5 M) in the superfusion medium to inhibit aminopeptidase(s) capable of inactivating AVP. Lastly, the stimulatory action of AVP on its own release was not blocked by a V1-receptor antagonist of AVP but was blocked by a V2-antagonist. It is proposed that this novel and robust positive feedback of AVP on its own release may be involved in the mechanism of memory consolidation of certain behavioral tasks known to be affected by AVP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2826.1990.tb00433.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Release of Vasopressin from the Septum and Hippocampus of Freely Behaving Male Rats: Effect of Bacitracin and Synthetic Vasopressin (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neuroendocrinology 2 (1990), S. 0 
    Details
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This paper describes the release characteristics of arginine vasopressin (AVP) from the dorsal septum and dorsal hippocampus of freely behaving male rats using miniaturized push-pull cannulae and a slow rate of perfusion to minimize tissue damage. The major findings were that) The spontaneous release of immunoreactive AVP (l-AVP) from the dorsal hippocampus was episodic in nature and did not require the presence of bacitracin in the perfusion medium to reliably demonstrate its pattern of release; 2) despite the fact that the septum has close to seven times more I-AVP per/mg of tissue, the dorsal septum perfusates had undetectable levels of the neuropeptide; 3) under these conditions hypertonic saline intraperitoneally did not alter the spontaneous release of this peptide; and 4) only when the perfusion medium contained effective concentrations of bacitracin (10−4 M) were reliable and detectable levels of I-AVP measured in perfusates from the dorsal septum and exogenous synthetic AVP had a robust positive feedback action on its own release. The physiological relevance and importance of this robust and novel effect of AVP remains to be elucidated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2826.1990.tb00432.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Push-Pull Perfusion of the Hypothalamus and the Caudate Nucleus in Conscious, Unrestrained Animals (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 473 (1986), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1986.tb23634.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A rapid and simple micromethod to measure picograms of Norepinephrine (NE) and Dopamine (DA) in small samples of brain tissue and pituitary gland (1976)
    Springer
    Journal of neural transmission 39 (1976), S. 291-300 
    Details
    ISSN: 1435-1463
    Keywords: DA ; NE ; radioenzymatic assay ; hypothalamic catecholamines ; pituitary catecholamines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A rapid and simple procedure to determine Dopamine (DA) and Norepinephrine (NE) in small tissue samples (μg quantities) is described. The radioenzymatic assay sensitivity, defined as twice the blank, is about 0.1 ng for NE and DA. Drugs known to modify brain catecholamine metabolism were shown to alter differentially DA and NE concentration in the medial basal hypothalamus (MBH) of male rats. Different areas of the brain and the pituitary gland, namely the parietal cortex, the pre-optic region (POA), the median eminence (ME), the medial basal hypothalamus without the median eminence and the posterior pituitary contain different concentrations of DA and NE, emphasizing the importance of regional analyses in tissue amines determination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01266305
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastructure of the rat median eminence after superfusion (1982)
    Springer
    Cell & tissue research 226 (1982), S. 27-35 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Ultrastructure ; In vitro systems ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Isolated medio-basal hypothalami of adult rats were continuously superfused in a chamber with controllable inputs and outputs, for periods from 30 to 240 min. The median eminence was prepared for transmission electron microscopy under carefully controlled conditions by immersion fixation with osmium tetroxide. The ultrastructure of superfused median eminence was compared with that of directly fixed, non-superfused median eminence. Even after 4h of superfusion, the median eminence displays remarkably well preserved histological and cytological patterns; cytomembranes, cell organelles, intercellular relationships, and extracellular spaces were remarkably similar in superfused and non-superfused tissues. As a consequence of osmium tetroxide fixation, microtubules were not observable. The ultrastructural information obtained from unstimulated rat median eminence superfused in vitro provides a basis for future morphofunctional correlations in the study of neurosecretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217079
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    Paper (German National Licenses)
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