ISSN:
1432-1912
Keywords:
Beagle dog large coronary arteries
;
5-HT1-like receptors
;
5-hydroxytryptamine
;
Sumatriptan
;
Model coronary spasm
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The effects of 5-hydroxytryptamine (5-HT), 5-carboxamidotryptamine (5-CT), methysergide and sumatriptan were studied on endothelium-denuded rings of beagle dog large coronary arteries. Submicromolar concentrations of the compounds contracted the rings with the order of potency 5-CT 〉 5-HT 〉sumatriptan = methysergide. Concentrations greater than 2 μM of both 5-HT and 5-CT, and 60 μmol/l methysergide also caused concentration-dependent relaxation. Sumatriptan did not cause relaxation. Peak intrinsic activities relative to the plateau contraction to sumatriptan (1.00), were 5-CT 0.47, 5-HT 0.87 and methysergide 0.51. Ketanserin 1 μmol/l affected neither contractile responses nor relaxant responses to 5-CT, methysergide and sumatriptan and only caused marginal blockade of the contractile effects of 5-HT. Methiothepin 200 nM shifted the concentration-contractile response curves by around 2 log units, as expected from its affinity for 5-HT1-like receptors. The rank order of contractile potency of the agonists, the antagonism by methiothepin and the resistance to blockade by ketanserin are consistent with a nearly exclusive involvement of 5-HT1-like receptors. Isolated large coronary arteries from beagle dogs may be a suitable model for the study of human coronary artery 5-HT1-like receptors that are involved in the spasm observed with 5-HT and sumatriptan.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00169018
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