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  • 1
    Electronic Resource
    Electronic Resource
    Configurations of glycosidic phosphates of lipopolysaccharide from Salmonella minnesota R595 (1982)
    s.l. : American Chemical Society
    Biochemistry 21 (1982), S. 6580-6586 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00268a040
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of Human Plasma Glycoproteins Separated by Two-Dimensional Gel Electrophoresis (1996)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 14 (1996), S. 66-70 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Purification of protein isoforms for the characterization of post-translational modifications, such as glycosylation, can be laborious and demanding. We report a means of determining monosaccharide composition and the identity of glycoproteins from a single spot on a two-dimensional (2-D) gel. The ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0196-66
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  • 3
    Electronic Resource
    Electronic Resource
    Flavones induce expression of nodulation genes in Rhizobium (1986)
    [s.l.] : Nature Publishing Group
    Nature 323 (1986), S. 632-635 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Infection of legumes by Rhizobium occurs mainly in the emerging root hair zone15. To demonstrate that this is the site of release of the substances that induce nod gene expression, seedlings were placed on a lawn of a R. trifolii strain containing lac genes from E. coli fused to the nodA gene (Fig. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/323632a0
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  • 4
    Electronic Resource
    Electronic Resource
    Fractionation of carbohydrates in Arabidopsis root cell walls shows that three radial swelling loci are specifically involved in cellulose production (2000)
    Springer
    Planta 211 (2000), S. 406-414 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Arabidopsis (cellulose ; mutants) ; Carbohydrate fractionation ; Cellulose synthesis ; Cell walls ; Mutant (Arabidopsis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract.  Three non-allelic radial swelling mutants (rsw1, rsw2 and rsw3) of Arabidopsisthaliana L. Heynh. were shown to be specifically impaired in cellulose production. Fractionation methods that identify, characterise and quantify some of the major cell wall polysaccharides in small quantities of seedlings demonstrated that changes in the production of cellulose are much more pronounced than changes in the production of non-cellulosic polysaccharides. A crude cell wall pellet was sequentially extracted with chloroform methanol (to recover lipids), dimethyl sulphoxide (starch), ammonium oxalate (pectins) and alkali (hemicelluloses). Crystalline cellulose remained insoluble through subsequent treatments with an acetic/nitric acid mixture and with trifluoroacetic acid. Cetyltrimethylammonium bromide precipitation resolved neutral and acidic polymers in the fractions, and precipitation behaviour, monosaccharide composition and glycosidic linkage patterns identified the major polysaccharides. The deduced composition of the walls of wild-type seedlings and the structure and solubility properties of the major polymers were broadly typical of other dicots. The three temperature-sensitive, radial swelling mutants produced less cellulose in their roots than the wild type when grown at their restrictive temperature (31 °C). There were no significant differences at 21 °C where no radial swelling occurs. The limited changes seen in the monosaccharide compositions, glycosidic linkage patterns and quantities of non-cellulosic polysaccharides support the view that the RSW1, RSW2 and RSW3 genes are specifically involved in cellulose synthesis. Reduced deposition of cellulose was accompanied by increased accumulation of starch.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250000301
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  • 5
    Electronic Resource
    Electronic Resource
    Five genetic loci involved in the synthesis of acidic exopolysaccharides are closely linked in the genome of Rhizobium sp strain NGR234 (1988)
    Springer
    Molecular genetics and genomics 212 (1988), S. 310-316 
    Details
    ISSN: 1617-4623
    Keywords: Exopolysaccharides ; Rhizobium NGR234 ; Tns mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary R-prime plasmids were constructed from a derivative of Rhizobium strain NGR234 (ANU280) and were shown to contain overlapping genomic DNA segments involved in biosynthesis of exopolysaccharides (EPS). The R-primes originally constructed carried the mutant allele from Tn5-induced EPS-deficient (Exo−) mutant ANU2811. This plasmid-located mutant allele was dominant to the corresponding wild-type allele as merodiploid strains were Exo−. Exo+ revertants occurred at a low rate (1×10-7) and these were shown to result from double reciprocal recombination events, which led to the isolation of R-prime plasmids carrying functional wild-type exo alleles. R-prime plasmids that carry overlapping segments of DNA from parental strain ANU280 complemented 28 of the 30 group 2 Exo− mutants of strain ANU280. Complementation of these Exo− mutants also restored their symbiotic abilities of effective nodulation. Subsequent in vivo recombination between the wild-type alleles located on the R-prime and the corresponding mutated allele on the genome, was used to generate a new family of R-primes, which carried mutations in the exo genes. The 30 group 2 Exo− mutants were classified into 7 distinct genetic groups based upon complementation and physical mapping data. Five of the seven exo loci were gentically linked and located on a 15-kb region of DNA. Mutations at two loci were dominant only when the mutations were R-prime plasmid-located while a mutation at a second locus was cis-dominant to two other exo loci. At least five genes involved in the synthesis of acidic exopolysaccharide synthesis have been identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334701
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  • 6
    Electronic Resource
    Electronic Resource
    A general approach to desalting oligosaccharides released from glycoproteins (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 737-747 
    Details
    ISSN: 1573-4986
    Keywords: desalting oligosaccharides ; graphitized carbon ; solid phase ; PNGase F ; electrospray mass spectroscopy ; HPAEC-PAD, high performance anion exchange chromatography with pulsed amperometric ; ESI-MS, electrospray ionization mass spectrometry ; PNGase F ; HexNAc, N-acetyl hexosamine ; Hex, hexose ; GalNAc4S,N-acetylgalactosamine-4-sulfate ; IduA, iduronic acid ; TFA ; SDS, sodium dodecyl sulfate ; NP40, Nonidet P40
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Desalting of sugar samples is essential for the success of many techniques of carbohydrate analysis such as mass spectrometry, capillary electrophoresis, anion exchange chromatography, enzyme degradation and chemical derivatization. All desalting methods which are currently used have limitations: for example, mixed-bed ion-exchange columns risk the loss of charged sugars, precipitation of salt by a non-aqueous solvent can result in co-precipitation of oligosaccharides, and gel chromatography uses highly crosslinked packings in which separation of small oligosaccharides is difficult to achieve. We demonstrate that graphitized carbon as a solid phase extraction cartridge can be used for the purification of oligosaccharides (or their derivatives) from solutions containing one or more of the following contaminants: salts (including salts of hydroxide, acetate, phosphate), monosaccharides, detergents (sodium dodecyl sulfate and Triton X-100), protein (including enzymes) and reagents for the release of oligosaccharides from glycoconjugates (such as hydrazine and sodium borohydride). There is complete recovery of the oligosaccharides from the adsorbent which can also be used to fractionate acidic and neutral glycans. Specific applications such as clean-up of N-linked oligosaccharides after removal by PNGase F and hydrazine, desalting of O-linked glycans after removal by alkali, on-line desalting of HPAEC-separated oligosaccharides and β-eliminated alditols prior to electrospray mass spectrometry, and purification of oligosaccharides from urine are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006983125913
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  • 7
    Electronic Resource
    Electronic Resource
    Binding of carbohydrates to solid supports Part 3: Reaction of sugar hydrazones with polystyrene substituted with aromatic aldehydes (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 847-854 
    Details
    ISSN: 1573-4986
    Keywords: sugar hydrazones ; sugar azines ; immobilization ; aminopolystyrene ; glycan modification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The hydrazones of glucose and N-acetylglucosamine, as models for the residues at the reducing termini of glycans, were covalently and reversibly bound in good yield to hydroxybenzaldehydo ligands attached to a polymer support. The binding, by a sugar azine linkage, occurred within two hours at room temperature at neutral pH, and efficient recoveries of sugars from the beads were achieved by displacement with aqueous hydrazine hydrate, ethanolic benzaldehyde, or aqueous acetone. Enzyme modification of glycans was demonstrated by separation of the products of hydrolysis of lactose hydrazone with β-galactosidase, using hydroxybenzaldehyde-derivatized polystyrene beads. Addition of a spacer arm to aminopolystyrene beads, for binding of reducing sugars as Amadori compounds to the aromatic amine function, was also investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006998114193
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  • 8
    Electronic Resource
    Electronic Resource
    The elimination ofO-linked glycans from glycoproteins under non-reducing conditions (1994)
    Springer
    Glycoconjugate journal 11 (1994), S. 163-167 
    Details
    ISSN: 1573-4986
    Keywords: O-linked sugars ; mucin ; glycoprotein ; β-elimination ; sugar hydrazones ; reducing sugars
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A simple procedure is described for the elimination ofO-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive β-elimination ofO-glycans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00731156
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  • 9
    Electronic Resource
    Electronic Resource
    Binding of carbohydrates to solid supports part 2: reaction of sugar hydrazones with isothiocyanate-substituted polystyrene (1994)
    Springer
    Glycoconjugate journal 11 (1994), S. 586-592 
    Details
    ISSN: 1573-4986
    Keywords: sugar hydrazones ; immobilization ; aminopolystyrene ; isothiocyanatopolystyrene ; thiosemicarbazone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The binding of sugars to a polymer support as thiosemicarbazones has been investigated as a means of immobilizing glycans. Hydrazones of glucose andN-acetylglucosamine were prepared by reaction with hydrazine hydrate, and successfully reacted with isothiocyanate-substituted polystyrene by incubation at room temperature and neutral pH. The binding was efficient and stable in aqueous buffers over a range of pH conditions. The bound sugars were recovered in moderate yield by treatment of the beads with hydrazine hydrate, benzaldehyde or acetone. Direct binding of reducing sugars to thiosemicarbazide-substituted polystyrene was not successful because of the unfavourable thermodynamics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00731310
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  • 10
    Electronic Resource
    Electronic Resource
    Binding of carbohydrates to solid supports: evaluation of a prototype system (1994)
    Springer
    Glycoconjugate journal 11 (1994), S. 11-16 
    Details
    ISSN: 1573-4986
    Keywords: reducing sugars ; immobilization ; Amadori rearrangement ; aminopolystyrene ; immunoassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Mono- and disaccharides were covalently and irreversibly bound to aminopolystyrene beads in good yield by heating in dilute aqueous solution. The degree and stability of sugar binding were determined by chemical and radiochemical methods and the accessibility of the bound sugars was demonstrated by exoglycosidase hydrolysis and by an enzyme-linked lectin-binding assay using Concanavalin A.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00732427
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