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  • 1
    Electronic Resource
    Electronic Resource
    Changes in serum parameters of Saprolegnia-infected brown trout, Salmo trutta L. (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 2 (1979), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Fungal infection of both wild and hatchery-reared sexually mature brown trout, Salmo trutta L., was caused by Saprolegnia diclina Humphrey Type 1. The major ions in the serum of infected fish were all significantly reduced in concentration and it is suggested that the primary cause of death was osmoregulatory breakdown resulting in a lethal haemodilution. Severe hypoproteinaemia and a significant reduction in the albumin/globulin ratio were reflected in the electro-phoretogram of the serum proteins from infected fish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1979.tb00159.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Antibiotic protection against recrudescence of latent Aeromonas salmonicida during furunculosis vaccination (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 19 (1996), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Problems of temporary immunosuppression following vaccination against Aeromonas salmonicida infection had to be overcome in the development of a furunculosis vaccine. Empirical observations have indicated that immunosuppression persists for some time after vaccination, rendering fish, especially subclinical carriers of A. salmonicida, highly vulnerable to bacterial invasion. The efficacy of simultaneous application of furunculosis vaccine and a long-lasting amoxycillin preparation to a population of Atlantic salmon smolts was evaluated. Control groups were treated with either vaccine alone, amoxycillin alone or were untreated. Moderate stress, simulating smolt transfer with a 5°C temperature rise, resulted in a rapid outbreak in mortalities reaching 100% in the vaccinates. Losses among the untreated controls were more gradual and rose to about 50%. Both amoxycillin-treated groups survived well. Further severe stress resulted in total mortalities among the untreated fish but no further losses in the amoxycillin groups. Four months after vaccination, evidence of a specific immune response was confirmed by ELISA, demonstrating circulating antibodies in the blood of vaccinates. In a severe and in a moderate challenge with A. salmonicida., the relative specific protection was 63 and 86%, respectively. Thus, effective protection against furunculosis was achieved without jeopardizing the stock during the vaccination process and with elimination of the carrier state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.1996.d01-86.x
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  • 3
    Electronic Resource
    Electronic Resource
    Current practices in the chemotherapeutic control of sea lice infestations in aquaculture: a review (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 16 (1993), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. This review surveys the available literature on the efficacy and ecotoxicology of chemotherapeutic agents for the control of sea lice (Copepoda: Caligidae) infestations in marine aquaculture. To date, treatment for the disease has relied almost exclusively on the use of the organophosphorus pesticides, trichlorfon and dichlorvos. While use of dichlorvos and trichlorfon appears to have little, if any, environmental impact, protracted use in some areas has led to reduced efficacy. Compounds proposed for sea lice control currently under field evaluation include azamethiphos (organophosphate), ivermectin (avermectin), pyrethrum (pyrethrin) and hydrogen peroxide. Although all of these compounds are effi cacious, at present published information on marine environmental toxicology is scarce and is limited to in vitro toxicity testing. Other compounds that have been considered for sea lice control include formalin, malathion (organophosphate), carbaryl (carbamate), diflubenzuron (insect growth regulator) and natural remedies (i.e. onions and garlic). In general, formaldehyde (aldehyde), malathion and natural compounds show either poor efficacy or unsuitable therapeutic margins. Although carbaryl and diflubenzuron are both efficacious and exhibit suitable therapeutic margins, available data suggests that the compounds, due to undesirable environmental toxicological characteristics, would be unsuitable for sea lice control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1993.tb00844.x
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of buffers on the gill structure of common carp, Cyprinus carpio L., and rainbow trout, Oncorhynchus mykiss (Walbaum) (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 18 (1995), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. The effects of phosphate and tris buffers on the gill structure of common carp, Cyprinus carpio L, and rainbow trout, Oncorhynchus mykiss (Walbaum), were investigated. Common carp maintained in phosphate-buffered (a combination of sodium di-hydrogen phosphate and di-sodium hydrogen phosphate at a final concentration of 0.01 m) standard dilution water for a period of 8 days developed severe pathological changes in the gill tissues, while carp maintained in tris-buffered (0.01 m) dilution water and trout subjected to either of these buffers showed no such changes. Histological changes in the gills of carp exposed to phosphate buffered dilution water included an extensive mucous cell proliferation on the lamellar and filarnental epithelium, and an increased infiltration of eosinophilic granular cells into the gill tissues. A moderate epithelial hyperplasia and an increase in the chloride cell densities on the gill epithelia were also noted. The implications of these findings on the widely accepted practice of the use of phosphate salts and various other buffers in pH control of experimental water are highlighted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1995.tb00333.x
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  • 5
    Electronic Resource
    Electronic Resource
    Some biochemical properties and antibiotic sensitivities of Pasteurella piscidda isolated in Greece and comparison with strains from Japan, France and Italy (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 18 (1995), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract The biochemical properties and antibiotic sensitivity patterns of 13 Pasteurella piscicida isolates from Greece are described and compared with 10 Japanese and five European (Italian and French) isolates. Morphologically and biochemically, all isolates of P. piscidda tested were nearly identical and only minor differences could be detected with reference to the level of acid production from sugars (especially from glucose). Greek, Italian and French isolates showed similar antibiotic sensitivity patterns, being resistant to erythromycin, kanamycin and streptomycin, and sensitive to most of the other antibiotics tested. Resistance was also demonstrated for some isolates towards the potentiated sulphonamides. The Japanese isolates appeared to be resistant to a larger range of antibiotics including erythromycin, kanamycin, streptomycin, oxolinic acid, oxytetracycline and chloramphenicol. Multiple resistance of Japanese strains was also noted. These results are discussed in the light of future measures for the control of pasteurellosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1995.tb01260.x
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  • 6
    Electronic Resource
    Electronic Resource
    A systemic protozoal disease of cultured salmonids (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 12 (1989), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Sporadic cases of a systemic protozoal infection with cells resembling Dermocystidium sp. are described from cultured rainbow trout, Salmo gairdneri Richardson, from France and brown trout, Salmo trutta L., and Atlantic salmon, Salmo salar L., from Ireland. Classical proliferative kidney disease was additionally found in the rainbow trout and PKX-like cells were also seen in the brown trout and salmon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1989.tb00288.x
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  • 7
    Electronic Resource
    Electronic Resource
    Further consideration of the haematology of proliferative kidney disease (PKD) in rainbow trout, Salmo gairdneri Richardson (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 10 (1987), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. A study of selected haematological parameters in rainbow trout, Salmo gairdneri Richardson, exposed to proliferative kidney disease (PKD) under field conditions is described. Changes in the blood picture are related to kidney pathology at different stages of the disease. As extensive renal vascular pathology, haemoglobin crystallization and inflammatory lesions developed, there were progressive decreases in haemoglobin and packed cell volume values, erythrocyte numbers declined to less than 50% of those in control fish, and numbers of misshapen erythrocytes increased. Leucopenia, neutrophilia and thrombocytopenia developed, especially in oedematous fish. With recovery, most values were similar to those of control fish. In recovered, re-challenged fish, erythrocyte counts, packed cell volume and haemoglobin values decreased in infected fish compared with uninfected fish. The results of the study are compared with those of other authors and the type of anaemia in PKD is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1987.tb01094.x
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  • 8
    Electronic Resource
    Electronic Resource
    Production and characterization of monoclonal antibodies against Pasteurella piscicida, the causative agent of fish pasteurellosis (1997)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 20 (1997), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Six monoclonal antibodies (mabs) produced against Pasteurella piscicida whole cells, cultured under iron limitation conditions, were characterized and specificity tested. Two of the mabs (WE15H1D7 and WE3D6C11) appeared to be specific for P. piscicida lipopolysaccharide, while mabs WE14C10F9 and WE12D7D8 crossreacted with Photobacterium species. The remaining two mabs, WE9D6D8 and VP4B11, crossreacted with other bacterial genera. All mabs reacted with material present in the extracellular products of the pathogen as well as to whole cells, suggesting that the antigens to which antibodies are directed slough off the cell membranes or are secreted to the culture medium. The potential of the mabs as tools for the diagnosis of pasteurellosis is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.1997.00298.x
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  • 9
    Electronic Resource
    Electronic Resource
    The effect of iron limitation growth conditions on the cell and extracellular components of the fish pathogen Pasteurella piscicida (1997)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 20 (1997), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The effect of iron limitation, using the iron-chelating agent 2,2 dipyridyl, on the electrophoretic profiles of outer membrane proteins (OMPs) and extracellular products (ECPs) from 21 Pasteurella piscicida strains isolated from Europe and Japan was investigated. In addition, the effect of iron-limited and iron-surplus growth conditions on caseinase activity in culture supernatants of the pathogen was examined. The majority of P. piscicida strains, from Greece, Italy and France, cultured under iron-limited conditions, produced four novel OMPs (63 and three at and above 200 kDa). In contrast, iron-regulated outer membrane proteins were not induced in Japanese strains. Electrophoretic analysis of the ECPs from the pathogen grown under iron surplus and iron limitation revealed a large range of products and additional high molecular mass (MM) bands were evident under iron-limited conditions. When culture supernatants were analysed for their activity, most of the bacteria tested showed elevated activities under iron limited conditions. Finally, neither hydroxamate nor phenolate type siderophores could be detected with any of the chemical assays used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.1997.00299.x
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  • 10
    Electronic Resource
    Electronic Resource
    Mortalities in Atlantic salmon, Salmo salar L., associated with pathology of the melano-macrophage and haemopoietic systems (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 15 (1992), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1992.tb00656.x
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