ISSN:
1432-0584
Keywords:
Bone marrow
;
Flow cytometry
;
Monoclonal antibodies
;
Hematopoiesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Bone marrow aspirates from 48 healthy donors (34 adults, 14 children) were analyzed by flow cytometry (FACS Analyzer) after purification of low-density bone marrow cells (Ld BMC) on a density gradient (d = 1,077) and labelling with 23 anti-hematopoietic cell monoclonal antibodies. Based on physical properties, these Ld BMC could be divided into four different populations called E, My, Mo and L, which comprised 14% ± 9%, 31% ± 16%, 10% ± 5% and 45% ± 14% of these cells, respectively. The phenotypic analysis of these different populations enabled the identification in E, of erythrocytes (Glycophorin A+, Rhesus D+, but negative for early erythroid differentiation markers such as the transferrin receptor (Tf. R) and the FA6-152 antigen); in My of cells of the myeloid lineage (VIM2+, HLA DR−); in Mo of cells of the monocytic lineage (VIM2+, CD14+) plus some myeloblasts (VIM2+, CD14−, HLADR+) and finally in L of a heterogeneous population including: 1. T lymphocytes labelled to the same extent by CD2, CD3, CD5 and CD6 (28% ± 10%), B lymphocytes assessed by CD19 and CD20 (12% ± 8%), Pre-B cells (CD10+ = 8% ± 7%), less than 5% of “natural killer” cells (CD16+ or Leu7+) and finally, less than 6% of myelomonocytes (CD14+ and/or VIM2+). 2. The erythroid lineage (rhesus D+ = 42% ± 20%, Tf.R+ and FA6-152+ = 32% ± 12%). 3. Undifferentiated cells or progenitor cells (CD34+ = 7% ± 5%). 4. Cells unlabelled by any antibodies (approximately 6%). We observed no difference between bone marrow samples from adults or children, with respect to physical properties, and with all but four immunological markers. A significantly higher proportion of B cells (CD19+ and CD10+) (P〈0.001) and undifferentiated cells (CD34+ and HLADR+) (P〈0.02) was observed in children. These data, obtained from a large number of bone marrow samples, could be used to quantify the imbalance of some bone marrow disorders.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01732876
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